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Sample GSM1006845 Query DataSets for GSM1006845
Status Public on Jul 29, 2013
Title brain_control_rep4
Sample type RNA
 
Source name 25 weeks, young control
Organism Mus musculus
Characteristics tissue: brain
strain: C57BL/6JRj
genotype: wildtype
age: 25 weeks
mouse id: 30210295-10
Treatment protocol Male C57BL6/J Rj mice were obtained from Janvier (France) and were randomly assigned to rapamycin or vehicle treatment groups. We had three treatment cohorts, distinguished by age of treatment onset. Treatment was initiated in young adulthood (i.e., at approx. 4 months of age), in midlife (i.e., at 13 months of age) or late in life (i.e., at 20-22 months of age). Animals were treated for >12 months before analyses started at 16 months, 25 months and 34 months of age, respectively. Animals were kept on their respective diet for the entirety of the study. In all cases, the experimenter was blinded to the animal group assignments.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 4
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Sep 20, 2012
Last update date Jul 29, 2013
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE41018 Rapamycin extends life span, but has limited effects on aging in mice

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1355.626 0
ILMN_2721178 342.883 0
ILMN_3033922 462.2608 0
ILMN_3092673 2103.231 0
ILMN_2816356 12.20878 0.02756892
ILMN_2808939 393.7309 0
ILMN_2634564 144.4116 0
ILMN_2737647 15.63079 0.01378446
ILMN_2734484 103.663 0
ILMN_2952292 8.968364 0.07017544
ILMN_2699078 -1.677146 0.5388471
ILMN_1213681 114.9078 0
ILMN_2735413 -0.4339281 0.4348371
ILMN_2735415 -0.2619744 0.4185464
ILMN_2891688 214.619 0
ILMN_2637698 558.5104 0
ILMN_2674228 102.885 0
ILMN_2601546 9.292824 0.0651629
ILMN_1230831 19.93377 0.007518797
ILMN_2848071 13.13199 0.02255639

Total number of rows: 25697

Table truncated, full table size 731 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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