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Sample GSM1006848 Query DataSets for GSM1006848
Status Public on Jul 29, 2013
Title brain_treated_rep4
Sample type RNA
 
Source name 25 month, rapamycin treated
Organism Mus musculus
Characteristics tissue: brain
strain: C57BL/6JRj
genotype: wildtype
age: 25 month
mouse id: 30210465-33
Treatment protocol Male C57BL6/J Rj mice were obtained from Janvier (France) and were randomly assigned to rapamycin or vehicle treatment groups. We had three treatment cohorts, distinguished by age of treatment onset. Treatment was initiated in young adulthood (i.e., at approx. 4 months of age), in midlife (i.e., at 13 months of age) or late in life (i.e., at 20-22 months of age). Animals were treated for >12 months before analyses started at 16 months, 25 months and 34 months of age, respectively. Animals were kept on their respective diet for the entirety of the study. In all cases, the experimenter was blinded to the animal group assignments.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 4
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
 
Submission date Sep 20, 2012
Last update date Jul 29, 2013
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE41018 Rapamycin extends life span, but has limited effects on aging in mice

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 1285.994 0
ILMN_2721178 325.1213 0
ILMN_3033922 412.0353 0
ILMN_3092673 2212.133 0
ILMN_2816356 4.393217 0.1704261
ILMN_2808939 419.0328 0
ILMN_2634564 177.9663 0
ILMN_2737647 14.21707 0.02255639
ILMN_2734484 111.5976 0
ILMN_2952292 19.49449 0.006265664
ILMN_2699078 -8.882136 0.9536341
ILMN_1213681 117.7098 0
ILMN_2735413 -4.02677 0.6992481
ILMN_2735415 3.872951 0.1842105
ILMN_2891688 212.9135 0
ILMN_2637698 588.4596 0
ILMN_2674228 84.8252 0
ILMN_2601546 3.14033 0.20802
ILMN_1230831 12.83585 0.03007519
ILMN_2848071 13.98749 0.02506266

Total number of rows: 25697

Table truncated, full table size 728 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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