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Sample GSM1006856 Query DataSets for GSM1006856
Status Public on Jul 29, 2013
Title heart_treated_rep2
Sample type RNA
Source name 25 month, rapamycin treated
Organism Mus musculus
Characteristics tissue: heart
strain: C57BL/6JRj
genotype: wildtype
age: 25 month
mouse id: 30210462-30
Treatment protocol Male C57BL6/J Rj mice were obtained from Janvier (France) and were randomly assigned to rapamycin or vehicle treatment groups. We had three treatment cohorts, distinguished by age of treatment onset. Treatment was initiated in young adulthood (i.e., at approx. 4 months of age), in midlife (i.e., at 13 months of age) or late in life (i.e., at 20-22 months of age). Animals were treated for >12 months before analyses started at 16 months, 25 months and 34 months of age, respectively. Animals were kept on their respective diet for the entirety of the study. In all cases, the experimenter was blinded to the animal group assignments.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol using HiScan
Description replicate 2
Data processing The data were normalised using cubic spline normalisation with Illumina Genomestudio 2011.1
Submission date Sep 20, 2012
Last update date Jul 29, 2013
Contact name Martin Irmler
Organization name Helmholtz Zentrum M√ľnchen GmbH
Department Institute of Experimental Genetics
Lab Gene Regulation & Epigenetics
Street address Ingolstaedter Landstrasse 1
City Neuherberg
State/province Bayern
ZIP/Postal code 85764
Country Germany
Platform ID GPL6885
Series (1)
GSE41018 Rapamycin extends life span, but has limited effects on aging in mice

Data table header descriptions
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 2540.439 0
ILMN_2721178 322.5593 0
ILMN_3033922 428.1479 0
ILMN_3092673 1857.12 0
ILMN_2816356 34.17172 0.002506266
ILMN_2808939 75.76785 0
ILMN_2634564 159.3328 0
ILMN_2737647 9.643927 0.08897243
ILMN_2734484 175.2436 0
ILMN_2952292 13.39205 0.05137845
ILMN_2699078 -3.067957 0.6641604
ILMN_1213681 531.4496 0
ILMN_2735413 1.955668 0.3408521
ILMN_2735415 -0.4499829 0.4837093
ILMN_2891688 466.2867 0
ILMN_2637698 627.138 0
ILMN_2674228 87.97263 0
ILMN_2601546 14.52795 0.04636591
ILMN_1230831 4.965425 0.2142857
ILMN_2848071 14.41155 0.04761905

Total number of rows: 25697

Table truncated, full table size 744 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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