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Sample GSM1007865 Query DataSets for GSM1007865
Status Public on Nov 15, 2012
Title 5388438026_B
Sample type RNA
 
Source name Dissected spine
Organism Mus musculus
Characteristics strain: BALB/c
genotype/variation: IL4 knockout
treatment: none (Control)
Extracted molecule total RNA
Extraction protocol Spines were dissected from 4 control and 4 PGISp-affected mice and flash frozen in liquid nitrogen. For the PGISp mice, only mice showing severe peripheral arthritis were selected.
RNA was extracted from the spines using Trizol as per the manufacturers protocol with the extra spin step included as recommended for bone samples then cleaned up using RNAeasy columns.
Label Cy3
Label protocol 500ng of RNA was used in the Illumina TotalPrep cRNA Amplification Kit according to the standard protocol. Samples were hybridised to Mouse Ref-8 Expression BeadChips.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description RNA extracted from flash frozen mice spines using Trizol
Data processing Array data were processed using the Illumina BeadStudio software then the processed data were assessed for quality control and normalised in Lumi. Analysis of gene expression patterns was performed in BRB-ArrayTools. For quality control scanned images of the arrays were visually inspected for artefacts in Illumina BeadStudio followed by the graphical analysis of density plots in Lumi. The microarray data were transformed by variance stabilization transformation (VST) then normalized by robust spline normalization (RSN). Post-normalization quality control was carried out using density plots to ensure all samples had similar distribution and variance. To reduce background noise in the data analysis all probes that were not expressed in any of the samples, i.e. whose intensity was below background in all samples, were excluded from the analysis. This did not exclude probes for genes whose expression was either “switched-on” or “switched-off”.
 
Submission date Sep 20, 2012
Last update date Nov 15, 2012
Contact name Gethin Thomas
E-mail(s) gethin.thomas@uq.edu.au
Phone +61 7 3176 2755
Fax +61 7 3176 5946
Organization name University of Queensland Diamantina Institute
Street address Princess Alexandra Hospital
City Woolloongabba
State/province QLD
ZIP/Postal code 4102
Country Australia
 
Platform ID GPL6885
Series (1)
GSE41039 Control vs. PGISp spine samples

Data table header descriptions
ID_REF
VALUE transformed by variance stabilization transformation (VST) then normalized by robust spline normalization (RSN)

Data table
ID_REF VALUE
ILMN_2896528 10.35120106
ILMN_2721178 8.787965775
ILMN_3033922 8.2345438
ILMN_3092673 9.867049217
ILMN_2816356 6.816162586
ILMN_2808939 7.837341785
ILMN_2634564 7.946931839
ILMN_2737647 6.627346516
ILMN_2734484 7.931934357
ILMN_2952292 6.86647892
ILMN_2699078 6.679555416
ILMN_1213681 7.445163727
ILMN_2735413 6.603677273
ILMN_2735415 6.698773861
ILMN_2891688 8.418251038
ILMN_2637698 8.699380875
ILMN_2674228 7.616408348
ILMN_2601546 6.611250877
ILMN_1230831 6.618923187
ILMN_2848071 6.69556427

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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