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Status |
Public on Feb 28, 2013 |
Title |
wild type zebrafish liver_biological rep3 |
Sample type |
RNA |
|
|
Source name |
The adult livers of 3-month-old wild type zebrafish
|
Organism |
Danio rerio |
Characteristics |
strain: AB genotype: Wild type gender: male age: 3-month-old tissue: liver
|
Growth protocol |
Zebrafish were raised and maintained in the standard Zebrafish Unit under a constant 14 hour on/10 hour off light cycle at 28℃.
|
Extracted molecule |
total RNA |
Extraction protocol |
Zebrafish were anesthetized in Tricaine solution and dissected for the non-fixed liver tissue. The tissues were put into the Trizol immediately and passed through 23-26 G needle for homogenization. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Samples were amplified and labeled using the 3' IVT Express Kit (Affymetrix)
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|
|
Hybridization protocol |
Samples were hybridized with the GeneChip® Zebrafish Genome Array for 16 h, followed by washed and stained with the Affymetrix fluidics station 450.
|
Scan protocol |
GeneChips were scanned with the Affymetrix 3000 7G plus scanner.
|
Description |
Gene expression data from the adult livers of 3-month-old wild type zebrafish
|
Data processing |
Dat and Cel files were obtained by the AGCC software, and the CHP files were generated with MAS5.0 method by Affymetrix Expression Console.
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Submission date |
Sep 21, 2012 |
Last update date |
Feb 28, 2013 |
Contact name |
Jinrong Peng |
E-mail(s) |
pengjr@zju.edu.cn
|
Organization name |
Zhejiang University
|
Street address |
866 Yuhangtang Road
|
City |
Hangzhou |
ZIP/Postal code |
310029 |
Country |
China |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE41059 |
Expression profiles of zebrafish transgenic line Tg(fabp10a:def)-I |
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