|
| Status |
Public on Feb 28, 2013 |
| Title |
Tg(fabp10a:def)-I liver_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
The adult livers of 3-month-old Tg(fabp10a:def)-I transgenic line
|
| Organism |
Danio rerio |
| Characteristics |
strain: AB
genotype: Tg(fabp10a:def)-I
gender: male
age: 3-month-old
tissue: liver
|
| Growth protocol |
Zebrafish were raised and maintained in the standard Zebrafish Unit under a constant 14 hour on/10 hour off light cycle at 28℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Zebrafish were anesthetized in Tricaine solution and dissected for the non-fixed liver tissue. The tissues were put into the Trizol immediately and passed through 23-26 G needle for homogenization.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were amplified and labeled using the 3' IVT Express Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized with the GeneChip® Zebrafish Genome Array for 16 h, followed by washed and stained with the Affymetrix fluidics station 450.
|
| Scan protocol |
GeneChips were scanned with the Affymetrix 3000 7G plus scanner.
|
| Description |
Gene expression data from the adult livers of 3-month-old Tg(fabp10a:def)-I transgenic line
|
| Data processing |
Dat and Cel files were obtained by the AGCC software, and the CHP files were generated with MAS5.0 method by Affymetrix Expression Console.
|
| |
|
| Submission date |
Sep 21, 2012 |
| Last update date |
Feb 28, 2013 |
| Contact name |
Jinrong Peng |
| E-mail(s) |
pengjr@zju.edu.cn
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yuhangtang Road
|
| City |
Hangzhou |
| ZIP/Postal code |
310029 |
| Country |
China |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE41059 |
Expression profiles of zebrafish transgenic line Tg(fabp10a:def)-I |
|
