|
| Status |
Public on Jan 01, 2013 |
| Title |
Sox17-GFP(+) from Nodal treatment |
| Sample type |
RNA |
| |
|
| Source name |
Sox17-GFP mESC_Nodal_7d
|
| Organism |
Mus musculus |
| Characteristics |
strain background: 129
genotype/variation: Sox17-GFP
cell type: mESC
|
| Treatment protocol |
Endoderm differentiation was induced for 6-8 days in DMEM, 5% FBS, 0.1mM NEAA, 1x Glutamax, 1x Penn/Strep, 0.055mM βMe, supplemented with 50 ng/ml recombinant human Activin A or 1000ng/ml recombinant mouse Nodal; media changed every other day.
|
| Growth protocol |
Mouse ES cells were maintained on gelatin-coated plates with MEFs in DMEM, 0.1mM NEAA, 1x Glutamax, 1x Penn/Strep (Invitrogen), 15% FBS (HyClone), 0.055mM 2-mercaptoethanol (βMe; Sigma), and 5x10^5 units LIF (Chemicon)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated using Qiashredder and RNAeasy Mini Kit. Biotinylated cRNA prepared from ≥100ng of RNA using Illumina TotalPrep RNA Amplification Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
3 replicates for each sample
|
| Data processing |
The data were normalized using cubic spline with Bead Studio v2.0
|
| |
|
| Submission date |
Sep 22, 2012 |
| Last update date |
Jan 01, 2013 |
| Contact name |
Alice E Chen |
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology
|
| Lab |
Douglas Melton
|
| Street address |
7 Divinity Avenue
|
| City |
Cambridge |
| State/province |
Ma |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE41086 |
Gene expression profiling of Nodal versus Activin-derived endoderm from mouse ES cells |
|
