|
| Status |
Public on Nov 20, 2012 |
| Title |
C57BL6_Control2 |
| Sample type |
RNA |
| |
|
| Source name |
Non-infected C57BL mouse control
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Cardiac tissue
|
| Treatment protocol |
Hearts of mice were extracted at day 18 post-infection with T cruzi.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled RNA fragments were prepared according to the standard protocol (Expression Anakysis Technical Manual, 2005, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on GeneChip Mouse Genome 430A 2.0. GeneChips were washed and stained in a fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from hearts of uninfected mice
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method, and confirmation by analysis using RMA from the Bioconductor affy package. MAS5 data was used to form the conclusions of this study (RMA was for secondary confirmation only).
|
| |
|
| Submission date |
Sep 22, 2012 |
| Last update date |
Nov 20, 2012 |
| Contact name |
Ricardo Gazzinelli |
| Organization name |
University of Massachussetts Medical School
|
| Department |
Infectious Diseases and Immunology
|
| Street address |
364 Plantation Street, LRB
|
| City |
Worcester MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE41089 |
Expression data from hearts of wild-type C57BL/6 mice infected with T. cruzi and controls (uninfected) |
|
