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Sample GSM101286 Query DataSets for GSM101286
Status Public on Mar 24, 2006
Title minus RA treatment replicate 2
Sample type RNA
 
Source name E14TG2a mouse embroyonic stem cells (ATCC, CRL-1841)
Organism Mus musculus
Characteristics mouse embryonic stem cell
Extracted molecule total RNA
Extraction protocol Total RNA from Mouse embryonic stem cells without all-trans retinoic acid induction were extracted using TRIzol reagent (Invitrogen) and further purified by Rneasy column (Qiagen;#75144).
Label biotin
Label protocol First & Second strand cDNA synthesis, cRNA Biotin-labelling and cRNA purification were performed using the Illumina TotalPrepRNA Amplification kit (Ambion;#IL1791). 850ng labeled cRNA were mixed with Hyb Mix (Hyb E1+Formamide) as according to instructions in BeadStation 500X Gene Expression System (Illumina) and used for microarray (Illumina Mouse Refseq-8 Array) staining.
 
Hybridization protocol All Hybridisation and Wash procedures were performed following instructions in the Experienced User Card #2, Hybridize/Wash 8-sample BeadChip protocol (Illumina). Briefly, 34ul of the Hyb mix with labeled cRNA sample was dispensed onto the center of each array and allowed to hyb with rotations in a pre-heated oven for 16-20h at 55*C. Washes were done with Wash E1BC solution, Block E1 solution was used for blocking and detection performed by Block E1 solution+Streptavidin-Cy3/5.
Scan protocol standard Illumina procedures
Description minus RA treatment replicate 2
Data processing RMA normalization
 
Submission date Mar 21, 2006
Last update date Mar 21, 2008
Contact name Bing Lim
E-mail(s) limb1@gis.a-star.edu.sg
Phone +65 64788156
Fax +65 64789005
URL http://www.gis.a-star.edu.sg
Organization name Genome Institute of Singapore
Department Stem Cell and Developmental Biology
Lab Stem Cell and Developmental Biology
Street address 60 Biopolis street, #02-01 Genome
City Singapore
State/province Singapore
ZIP/Postal code 138672
Country Singapore
 
Platform ID GPL4865
Series (1)
GSE4522 MicroRNA-134 Modulates Mouse Embryonic Stem Cell Differentiation

Data table header descriptions
ID_REF illumina ProbeID
VALUE illumina rank invariant normalized signal value
DET_VALUE illumina detection probability

Data table
ID_REF VALUE DET_VALUE
scl00227525.1_330-S -6.7 0.41616405
scl0003883.1_511-S 10.5 0.73220748
scl16326.12.4_133-S -2.4 0.51025332
scl00269955.1_25-S 9.2 0.71773221
scl0011717.2_283-S -0.1 0.56453559
scl52892.1.1_103-S -37.3 0.00844391
scl0012946.2_3-S 17.6 0.81423402
scl33480.16_131-S 31.5 0.89505428
scl0018762.1_190-S 297.2 1
scl00063.1_124-S -35.8 0.01085645
scl45217.22.1_37-S 44.9 0.94933655
scl056379.2_58-S -18.4 0.17852835
scl0268448.7_23-S 127.7 1
scl44375.15.1_26-S -0.3 0.56091677
scl0018933.1_65-S -10.5 0.32569361
scl0026384.1_20-S 54.1 0.97225573
scl29237.16.1_12-S -0.4 0.55971049
scl0074102.2_20-S -3 0.49819059
scl18491.1.2_327-S 3.7 0.64294331
scl16334.7.1_2-S 769.3 1

Total number of rows: 24044

Table truncated, full table size 757 Kbytes.




Supplementary data files not provided

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