|
| Status |
Public on Jul 28, 2006 |
| Title |
Lung_exposure 300µgTPM/l_2hrec B |
| Sample type |
RNA |
| |
|
| Source name |
lung tissue from rats exposed to 300µgTPM/l
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain: Sprague-Dawley
Gender: male
Tissue: lung
Exposition: 13weeks, 5d/week, 2h/day 300µgTPM/l
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with an additional second clean-up (Qiagen RNeasy Mini Kit)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8-11 microg total RNA (Expression Analysis Technical Manual, 701021 rev1, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Expression Array 230 A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner.
|
| Description |
Gene expression data from rat lungs, dissection occured after 13 weeks of exposition and 2hrs after last treatment
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 22, 2006 |
| Last update date |
Mar 22, 2007 |
| Contact name |
Jürgen Borlak |
| E-mail(s) |
borlak@item.fraunhofer.de
|
| Phone |
0049-511-5350520
|
| Organization name |
Fraunhofer Institut für Toxikologie und Experimentelle Medizin
|
| Street address |
Nikolai-Fuchs-Straße 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL342 |
| Series (1) |
| GSE4516 |
Gene expression profiles in lung tissue of rats following exposure to mainstream cigarette smoke |
|
