endothelial cells from wildtype newborn mice that were cultured in DMEM supplemented with 10% FCS, 2mM glutamine and 1x penicillin/streptomycin
Treatment protocol
soluble sTNF 4 hour treatment
Extracted molecule
total RNA
Label
biotin
Description
Gene expression data from endothelial cells from wildtype newborn mice transfected with treated for four hours with soluble TNF-alpha for transient activation. The cells were cultured in DMEM supplemented with 10% FCS, 2mM glutamine and 1x penicillin/streptomycin. Targets were produced using standard Affymetrix procedures from about 5ug of total RNA.
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
