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Sample GSM1015328 Query DataSets for GSM1015328
Status Public on Oct 17, 2012
Title Ex-4-mTBI-2
Sample type RNA
 
Source name Mus musculus hippocampus tissue
Organism Mus musculus
Characteristics tissue: Hippocampus
gender: Male
strain: ICR
weight: 30–40 g
treatment protocol: mice subjected to mTBI induced by weight drop injury while being treated with EX-4 (Ex-4/mTBI)
time: 14 days following mTBI
Treatment protocol Mice were treated for 7 days with the peptide Ex-4 dissolved in an equal volume of saline and dimethyl sulfoxide (DMSO) mixture and delivered from a subcutaneously implanted ALZET Micro-osmotic pump (Model 1007D, Alzet, Cupertino, CA) at a rate of 3.5 pM/kg/min. mTBI was induced on day 3 of treatment.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using the Qiagen RNeasy Mini Kit (Qiagen, Inc. Valencia CA) following the manufacturer's specifications. Quantity and quality of the RNA was assessed with the Agilent 2100 Bioanalyzer using RNA 6000 Nano Chips.
Label Streptavidin-Cy3 bound to biotin-labeled cRNA.
Label protocol standard Illumina protocol using Illumina TotalPrep RNA Amplification Kit (Ambion; Austin, TX, cat # IL1791) In short, 0.5g of total RNA was first converted into single-stranded cDNA with reverse transcriptase using an oligo-dT primer containing the T7 RNA polymerase promoter site and then copied to produce double-stranded cDNA molecules. The double stranded cDNA was cleaned and concentrated with the supplied columns and used in an overnight in-vitro transcription reaction where single-stranded RNA (cRNA) was generated and labeled by incorporation of biotin-16-UTP.
 
Hybridization protocol standard Illumina protocol. In short, a total of 0.75ug of biotin-labeled cRNA was hybridized at 58 degrees C for 16 hours to Illumina's Sentrix MouseRef-8 v2 Expression BeadChips (Illumina, San Diego, CA). Each BeadChip has ~24,000 well-annotated RefSeq transcripts with approximately 30-fold redundancy. The arrays were washed, blocked and the biotin labeled cRNA was detected by staining with streptavidin-Cy3.
Scan protocol Arrays were scanned at a resolution of 0.8um using the Beadstation 500 X from Illumina.
Description Hippocampus tissue of mice subjected to mTBI while being treated with EX-4.
Data processing Data was extracted using the Illumina BeadStudio software(v3.4.0). Any spots at or below the background were filtered out using an Illumina detection p value of 0.02 and above. The natural log of all remaining scores were used to find the avg and std of each array and the z-score normalization was calculated and presented below. Z-score = (raw value - avg)/std. Complete data including detection scores are included in the supplementary file linked to the Series record.
 
Submission date Oct 04, 2012
Last update date Jun 22, 2020
Contact name Supriyo De
Organization name NIA-IRP, NIH
Department Laboratory of Genetics and Genomics
Lab Computational Biology & Genomics Core
Street address 251 Bayview Blvd
City Baltimore
State/province Maryland
ZIP/Postal code 21224
Country USA
 
Platform ID GPL6885
Series (1)
GSE41345 Exendin-4, a glucagon-like peptide-1 receptor agonist prevents mTBI-induced changes in hippocampus gene expression and memory deficits in mice

Data table header descriptions
ID_REF
VALUE Z_VALUE (Z transformation of the natural log of the raw intensity values)
RAW_VALUE raw intensity value from Illumina BeadStudio software.

Data table
ID_REF VALUE RAW_VALUE
ILMN_1212607 -0.437828067 142.7278
ILMN_1212612 1.632387494 2246.032
ILMN_1212619 0.163970323 318.0108
ILMN_1212628
ILMN_1212632 -0.045642734 240.5763
ILMN_1212636 2.035876853 3843.224
ILMN_1212637 0.747748982 691.7624
ILMN_1212645 0.685245462 636.5319
ILMN_1212648 0.41069497 441.6591
ILMN_1212653 1.31135613 1464.906
ILMN_1212672 0.392882194 431.3091
ILMN_1212682
ILMN_1212683
ILMN_1212685
ILMN_1212692
ILMN_1212693 -0.316818162 167.6761
ILMN_1212695 -0.524807814 127.122
ILMN_1212698
ILMN_1212702 2.872992859 11713.3
ILMN_1212703 -0.360065954 158.295

Total number of rows: 25697

Table truncated, full table size 653 Kbytes.




Supplementary data files not provided
Raw data included within Sample table
Raw data are available on Series record
Processed data included within Sample table

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