|
| Status |
Public on Mar 24, 2006 |
| Title |
Drosophila heads_Fast group_biological rep1_RD01D013 |
| Sample type |
RNA |
| |
|
| Source name |
Heads of Drosophila received humidified air for 50 min followed by a single exposure of 50% ethanol vapor for 40 min.
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Genotype: Canton-S
Age: 2-5 days
|
| Treatment protocol |
Drosophila was treated with humidified air for 50 min followed by a single exposure of 50% ethanol vapor for 40 min, then flash frozen in liquid nitrogen.
|
| Growth protocol |
Flies were maintained on a 12 hr dark/12 hr light cycle on standard Drosophila medium at 25°C and 60% relative humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole heads of Canton-S flie were seperated from bodies using liquid nitrogen freezing and sieve (0.710 mm) seperation. About 60 seperated whole fly heads were homoginized in 0.5 ml Trizol (Invitrogen). Then 0.1 ml chloroform was added. After vigorous vortexing and centrifugation at 12,000 g for 15 minuntes, 0.28 ml of the aquous phase sample was transfered to a new tube, mixed with 0.25 ml 2-isopropanol, then loaded on a RNeasy column (Qiagen), the RNA sample was further purified by the RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from whole heads of flies treated with humidified air for 50 min followed by a single exposure of 50% ethanol vapor for 40 min
|
| Data processing |
The raw data of the 18 microarray were imported to dChip program (version 1.3). The arrays were normalized to a common baseline array that has the median overall brightness. The expression value for each probe set on each chip was then calculated as model-based expression index (MBEI) by Perfect Match-only model.
|
| |
|
| Submission date |
Mar 24, 2006 |
| Last update date |
Mar 24, 2006 |
| Contact name |
Zhiyong Yang |
| E-mail(s) |
zhiyongyang06@yahoo.com
|
| Phone |
832-326-1841
|
| Organization name |
Baylor College of Medicine
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE4531 |
Fast and chronic ethanol tolerance - time course |
|
