|
| Status |
Public on Sep 08, 2006 |
| Title |
Jurkat+Globin, cDNA target, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Jurkat cells, hemoglobin spikes added
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: T-lymphocyte leukemia cell line
|
| Biomaterial provider |
Affymetrix
|
| Extracted molecule |
total RNA |
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared using the Ovation Biotin System (NuGEN Technologies, Inc.) from 50 ng starting RNA.
|
| |
|
| Hybridization protocol |
cDNA was hybridized according to the Ovation Biotin RNA Amplification and Labeling System User Guide (rev. 1, NuGEN Technologies). Briefly, cDNA was hybridized at 10 ng/ul for 18 hours at 45 C.
|
| Scan protocol |
Arrays were scanned using a GeneChip Scanner 3000.
|
| Description |
Jurkat + Globin RNA, from GeneChip Globin-Reduction RNA Controls (Affymetrix P/N 900586)
|
| Data processing |
The data were analyzed with the MAS 5 algorithm in the R statistical environment using default settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 24, 2006 |
| Last update date |
Nov 04, 2009 |
| Contact name |
Aron C Eklund |
| Organization name |
Technical University of Denmark
|
| Department |
Center for Biological Sequence Analysis
|
| Street address |
Kemitorvet, Building 208
|
| City |
Lyngby |
| ZIP/Postal code |
2800 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE4532 |
Cross-hybridization comparison between cRNA target and cDNA target |
|
