|
| Status |
Public on Sep 03, 2013 |
| Title |
mRNA, mouse postnatal 6 week, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissue, mouse postnatal, 6 week, biological rep 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: whole lung
age: 6 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 500ng total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent 8X60K microRNA array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60K array slides
|
| Description |
gene expression data from lung tissue of postnatal mice
6wk_3
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters.
|
| |
|
| Submission date |
Oct 09, 2012 |
| Last update date |
Sep 03, 2013 |
| Contact name |
Kusum Vijay Pandit |
| E-mail(s) |
panditkusum@yahoo.com
|
| Phone |
412-624-9046
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine-PACCM
|
| Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE41412 |
Gene expression data in mouse fetal lungs |
|
