|
Status |
Public on Sep 03, 2013 |
Title |
mRNA, mouse postnatal day1, biological rep 5 |
Sample type |
RNA |
|
|
Source name |
Lung tissue, mouse postnatal, day1, biological rep 5
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: whole lung age: 1 day
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
Label |
Cy3
|
Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 500ng total RNA
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|
|
Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent 8X60K microRNA array. Arrays were washed according to the manufacturer's protocol.
|
Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60K array slides
|
Description |
gene expression data from lung tissue of postnatal mice d1_5
|
Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters.
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|
|
Submission date |
Oct 09, 2012 |
Last update date |
Sep 03, 2013 |
Contact name |
Kusum Vijay Pandit |
E-mail(s) |
panditkusum@yahoo.com
|
Phone |
412-624-9046
|
Organization name |
University of Pittsburgh
|
Department |
Medicine-PACCM
|
Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15213 |
Country |
USA |
|
|
Platform ID |
GPL10787 |
Series (1) |
GSE41412 |
Gene expression data in mouse fetal lungs |
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