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Status
Public on Mar 28, 2006
Title
HepG2-Neo-F4_Day 8
Sample type
RNA
Source name
pCMV transfected HepG2 cell, cultured for 8 days
Organism
Homo sapiens Characteristics
Plasmid pCMV transfected HepG2 cells, cultured for 8 days before array assay.
Growth protocol
Maintained in Dulbeccco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 250µg/ml G418, 100U/ml penicillin, 100µg/ml streptomycin, 2mM glutamine, 25mM HEPES solution, and 1mM sodium pyruvate at 37℃ under 5% CO2.
Extracted molecule
total RNA
Extraction protocol
Standard extraction protocol according to the TRIzol reagent manual.
Label
Biotin
Label protocol
Standard Affymetrix labelling protocol
Hybridization protocol
Standard Affymetrix hybridization protocol
Scan protocol
Standard Affymetrix scanning protocol
Description
Plasmid pCMV was transfected into HepG2 cells. After screened by G418, stable cell clones were selected and used for array.
Data processing
Affymetrix
Submission date
Mar 24, 2006
Last update date
Mar 27, 2006
Contact name
Xiaochen Tian
E-mail(s)
xiaochentian@hotmail.com Phone
86-21-54237572
URL
http://mvlab-fudan.cn/ Organization name
Fudan University
Department
Shanghai Medical college
Lab
lab of medical molecular virology
Street address
No.138 Yixueyuan Road
City
Shanghai
ZIP/Postal code
200032
Country
China
Platform ID
GPL570 Series (1)
GSE4549 Global exploration of interaction between HBsAg expression and Hepatocytes
Data table header descriptions ID_REF VALUE A measure of the relative abundance of a transcript.
ABS_CALL A measurement indicating if the transcript was detected (Present), not detected (Absent), or marginally detected (Marginal). Generally, p-value<0.04 is Present; 0.04<p-value<0.06 is Marginal; p-value>0.06 is Absent.
DETECTION P-VALUE A Detection p-value measures the probability that the discrimination scores of all probe pairs in the probe set are above a certain level, and that the target is likely to be Present.
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
126.3
P
0.00039
AFFX-BioB-M_at
199
P
0.00006
AFFX-BioB-3_at
121.5
P
0.000258
AFFX-BioC-5_at
244.8
P
0.000052
AFFX-BioC-3_at
243.9
P
0.000044
AFFX-BioDn-5_at
888.2
P
0.000044
AFFX-BioDn-3_at
1669.9
P
0.000044
AFFX-CreX-5_at
3393.6
P
0.000044
AFFX-CreX-3_at
3984.3
P
0.000044
AFFX-DapX-5_at
5.7
A
0.313723
AFFX-DapX-M_at
5.4
A
0.48511
AFFX-DapX-3_at
1.2
A
0.814869
AFFX-LysX-5_at
5.6
A
0.287743
AFFX-LysX-M_at
0.6
A
0.910541
AFFX-LysX-3_at
11.3
A
0.123572
AFFX-PheX-5_at
5.8
A
0.287743
AFFX-PheX-M_at
1.5
A
0.876428
AFFX-PheX-3_at
10.3
A
0.425962
AFFX-ThrX-5_at
1.4
A
0.749204
AFFX-ThrX-M_at
0.5
A
0.932322
54675 1393 Kbytes .Supplementary data files not provided
