|
| Status |
Public on Apr 30, 2006 |
| Title |
Dark rearing, biological rep2 CHC DR-2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse primary visual cortex (V1)
|
| Organism |
Mus musculus |
| Characteristics |
129/SvEv male and female mice P27 primary visual cortex
animals reared in darkness until P27
|
| Treatment protocol |
We followed the Eukaryotic Target Preparation” protocol as described in the Affymetrix manual.
|
| Growth protocol |
Animals came from different litters and the tissue was derived from primary visual cortex (V1) of at least two different animals. In both groups of animals, monocular and binocular portions were included for analysis. Mice were anesthetized with Nembutal before tissue extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Followed the “Eukaryotic Target Preparation” Affymetrix manual.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 15 to 45 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix hybridization protocol
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from mouse primary visual cortex
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
|
| |
|
| Submission date |
Mar 25, 2006 |
| Last update date |
Jun 15, 2006 |
| Contact name |
gabriel kreiman |
| E-mail(s) |
kreiman@mit.edu
|
| Phone |
6172539547
|
| URL |
http://www.mit.edu/~kreiman/
|
| Organization name |
MIT
|
| Department |
BCS
|
| Street address |
77 Massachusetts Avenue MIT 46-5155
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL83 |
| Series (1) |
| GSE4537 |
Expression data from mouse primary visual cortex under different deprivation conditions |
|
