NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1017533 Query DataSets for GSM1017533
Status Public on Sep 17, 2013
Title TiOblast6 DAY3
Sample type RNA
 
Source name implant adherent cells-alveolar bone-microroughosurface implant-day3
Organism Homo sapiens
Characteristics gender: Female
Treatment protocol Fourty-four implants (cp titanium) were placed in the alveolar bone of 11 systemically healthy human subjects missing 2 or more teeth.Following anesthesia(Lidocaine 2% with epinephrine (1:100,000)), two separate crestal incisions approximately 6mm in length were made and full thickness mucoperiosteal flaps were raised. Each subject received two TIO and two OS implants. Two osteotomies were prepared per site to allow placement of one implant of each surface (1 TiO and 1 Os). This paired surgical design permitted the retrieval of the first 2 implants (1 of each surface) at the earlier time point without disturbing the healing of the other 2 implants. Drilling was completed using a 2.0 mm twist osteotomy drill under profuse irrigation with sterile saline. Single use osteotomy drills were used on each subject. All implants were placed by a self-tapping procedure and primary stability at the time of implant placement was insured. A submerged implant installation technique was used and the mucosal tissues were closed primarily with 4-0 chromic gut sutures
Growth protocol 11 Human subjects missing 2 or more teeth were included.Following anesthesia(Lidocaine 2% with epinephrine (1:100,000)), two separate crestal incisions approximately 6mm in length were made and full thickness mucoperiosteal flaps were raised. Each subject received two TIO and two OS implants. Two osteotomies were prepared per site to allow placement of one implant of each surface (1 TiO and 1 Os). This paired surgical design permitted the retrieval of the first 2 implants (1 of each surface) at the earlier time point without disturbing the healing of the other 2 implants. Drilling was completed using a 2.0 mm twist osteotomy drill under profuse irrigation with sterile saline. Single use osteotomy drills were used on each subject. All implants were placed by a self-tapping procedure and primary stability at the time of implant placement was insured. A submerged implant installation technique was used and the mucosal tissues were closed primarily with 4-0 chromic gut sutures.
Extracted molecule total RNA
Extraction protocol At 3 and 7 days following surgery, one surgical site was chosen at random, re-entered and the paired (One TIO and one OS) implants were removed by reverse threading. The implants were then immediately rinsed in cold PBS and then placed into 1000 ul of Tri-reagent (Invitrogen, Carlsbad, CA). Cell lysates were snap frozen, and stored at -80°C until further use. Total RNA was isolated from the lysates using the standard Tri-reagent protocol and collected by ethanol precipitation. This was followed by purification using RNeasy MinElute Clean up kit (Qiagen, Valencia, CA, USA). RNA was assessed for quality and quantity using a bioanalyzer (Agilent, Santa Clara, CA, USA) and nanodrop ND-1000 spectrophotometer (Nanodrop, Wilmington, DE) respectively.
Label Biotin
Label protocol Samples were processed and hybridized to the Affymetrix Human gene 1.1 ST Array at the UNC core facility following the manufacturer’s recommended protocols and reagents (Affymetrix, San Clara,CA). Labeling used Nugen Encore Biotin Module
 
Hybridization protocol The Encore Biotin Module V2-NuGen/Affymetrix Human gene 1.1 ST Array
Scan protocol Affymetrix GeneChip Scanner 3000 7G Plus with Autoloader
Data processing The data was analyzed using GeneSpring software v.12.0
 
Submission date Oct 10, 2012
Last update date Sep 17, 2013
Contact name Lyndon Cooper
Organization name UNC School of Dentistry
Department Prosthodontics
Lab Bone Biology and Implant Therapy Laboratory
Street address 330 Brauer Hall, CB#7450
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platform ID GPL11532
Series (1)
GSE41446 Early molecular assessment of osseointegration in humans

Data table header descriptions
ID_REF
VALUE Summarization algorithm (RMA)

Data table
ID_REF VALUE
7896736 9.187891
7896738 5.2393064
7896740 6.966935
7896742 7.703591
7896744 7.086899
7896746 10.373408
7896748 10.838178
7896750 7.5362544
7896752 11.31233
7896754 4.533463
7896756 5.116874
7896759 5.011772
7896761 5.145029
7896779 4.939941
7896798 5.706337
7896817 5.6715274
7896822 5.1917725
7896859 4.8281493
7896861 4.3924336
7896863 4.881813

Total number of rows: 28869

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM1017533_H6D3_TIO.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap