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Sample GSM1018523 Query DataSets for GSM1018523
Status Public on Mar 27, 2013
Title Spleen Mock infected 6h Rep 1 (SC84)
Sample type RNA
Source name Spleen Mock infected 6h
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: Spleen
gender: Female
Treatment protocol Mice were infected for 6h with 5 x 10^7 CFU of Streptococcus suis strain SC84 or mock-infected with bacterial growth medium (THB).
Growth protocol Bacterial culture was prepared by transferring 10 μl of 1/1,000 dilutions of 8-h cultures into 30 ml of THB, which was incubated for 16 h at 37°C without agitation. Stationary-phase bacteria were washed twice in PBS (pH 7.3). The bacterial pellet was then resuspended and adjusted to a concentration of 5 × 10^8 c.f.u. ml-1.
Extracted molecule total RNA
Extraction protocol RNA was homogenized from RNAlater-stabilized spleen tissus, extracted and DNAse I treated using QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label Biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 1
Data processing The data were first raw-filtered in order to identify and remove probesets that were not detected and then normalised using robust spline normalization with FlexArray v1.6
Submission date Oct 11, 2012
Last update date Mar 27, 2013
Contact name Claude Lachance
Organization name Bio-Rad
Street address 1329 Meyerside Drive
City Mississauga
State/province Ontario
ZIP/Postal code L5T 1C9
Country Canada
Platform ID GPL6885
Series (1)
GSE41522 Genome wide analysis of C57BL-6 mice infected with Chinese strain (SC84) of Streptococcus suis
Reanalysis of GSM1018507

Data table header descriptions
VALUE log2 robust spline normalized

Data table
ILMN_1212607 7.988080412
ILMN_1212612 8.027932107
ILMN_1212636 11.44866959
ILMN_1212637 9.342969644
ILMN_1212645 8.345332479
ILMN_1212648 9.087107791
ILMN_1212653 8.503739979
ILMN_1212672 8.748842457
ILMN_1212692 8.068617859
ILMN_1212693 8.939786664
ILMN_1212695 8.05558276
ILMN_1212702 14.67393618
ILMN_1212703 8.168113658
ILMN_1212717 8.116242899
ILMN_1212720 8.689563401
ILMN_1212759 9.246132292
ILMN_1212791 8.17819056
ILMN_1212794 9.354513509
ILMN_1212836 8.6615524
ILMN_1212894 8.791890001

Total number of rows: 13325

Table truncated, full table size 323 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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