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Sample GSM1020054 Query DataSets for GSM1020054
Status Public on Oct 18, 2013
Title antmir125a-1_Mouse Lung Epithelial Cells MLE-12_rep1
Sample type RNA
 
Source name Mouse Lung Epithelial cell MLE-12, biological rep 1
Organism Mus musculus
Characteristics cell line: Lung Epithelial cell line MLE-12
transfection: antmir125a
passage: 4
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3 labeled RNA was prepared according to the standard Agilent protocol from 100ng total RNA
 
Hybridization protocol Labeled RNA was hybridized for 17 hr at 75C on Agilent 8X15K gene expression array. Arrays were washed according to the manufacturer's protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x15K array slides
Description Gene expression data from MLE-12 after 24h transfection
Data processing The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters.
Cyclic lowess, data was normalized with AFFY package in R and was transformed to log2.
 
Submission date Oct 15, 2012
Last update date Oct 18, 2013
Contact name christian lacks lino cardenas
E-mail(s) clinocardenas@mgh.harvard.edu
Phone 4127993484
Organization name Massachusetts general hospital-Harvard medical school
Department division of cardiovascular
Lab Lindsay
Street address 185 Cambridge st Room 3224
City Boston
State/province Massachusetts
ZIP/Postal code 02114
Country USA
 
Platform ID GPL10787
Series (1)
GSE41607 Gene expression data in Mouse Lung Epithelial cells MLE-12 transfected with inhibitors and precursors for miR-30a, miR-30d, miR-23b, miR-125 and miR-337, miR-466a, miR466d, miR-476c, respectively

Data table header descriptions
ID_REF
VALUE normalized signal intensity

Data table
ID_REF VALUE
A_30_P01017425 7.359163062
A_30_P01017426 6.017532652
A_30_P01017427 4.7673628
A_30_P01017428 7.329072598
A_30_P01017429 2.120644377
A_30_P01017430 2.730017501
A_30_P01017431 2.727449367
A_30_P01017432 5.734994585
A_30_P01017433 4.869510542
A_30_P01017434 5.991384049
A_30_P01017435 2.098447926
A_30_P01017436 2.07240202
A_30_P01017437 3.467345916
A_30_P01017438 2.09727141
A_30_P01017439 6.825541002
A_30_P01017440 2.364731432
A_30_P01017441 3.463803978
A_30_P01017442 7.409362759
A_30_P01017443 10.66497232
A_30_P01017444 3.337749801

Total number of rows: 52095

Table truncated, full table size 1318 Kbytes.




Supplementary file Size Download File type/resource
GSM1020054_antmir125a-1.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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