|
| Status |
Public on Oct 18, 2013 |
| Title |
premir466a-2_Mouse Lung Epithelial Cells MLE-12_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Lung Epithelial cell MLE-12, biological rep 2
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Lung Epithelial cell line MLE-12
transfection: premir466a
passage: 4
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 100ng total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 75C on Agilent 8X15K gene expression array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x15K array slides
|
| Description |
Gene expression data from MLE-12 after 24h transfection
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.1.1 (Agilent) using default parameters.
Cyclic lowess, data was normalized with AFFY package in R and was transformed to log2.
|
| |
|
| Submission date |
Oct 15, 2012 |
| Last update date |
Oct 18, 2013 |
| Contact name |
christian lacks lino cardenas |
| E-mail(s) |
clinocardenas@mgh.harvard.edu
|
| Phone |
4127993484
|
| Organization name |
Massachusetts general hospital-Harvard medical school
|
| Department |
division of cardiovascular
|
| Lab |
Lindsay
|
| Street address |
185 Cambridge st Room 3224
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE41607 |
Gene expression data in Mouse Lung Epithelial cells MLE-12 transfected with inhibitors and precursors for miR-30a, miR-30d, miR-23b, miR-125 and miR-337, miR-466a, miR466d, miR-476c, respectively |
|
