NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1022220 Query DataSets for GSM1022220
Status Public on Mar 19, 2014
Title Experiment1_si_YFP_3N (S)
Sample type RNA
 
Source name Mouse alveolar rhabdomyosarcoma tumor culture
Organism Mus musculus
Characteristics Stage: S
tranfection: 10nM siYFP
genotype/variation: Myf6Cre,Pax3:Foxo1a,p53 conditional aRMS mouse
Treatment protocol Cells were transfected by 10nM siYFP or control siRNA with 0.2% lipofectamine2000
Growth protocol DMEM+10%FBS
Extracted molecule total RNA
Extraction protocol Method of RNA isolation: Rnsasy Total RNA kit (Qiagen)
Label biotin
Label protocol Ambion MessageAmp Premier
 
Hybridization protocol Illumina MouseRef-8 v 2.0 protocol
Scan protocol Illumina MouseRef-8 v 2.0 protocol
Description 1siY3N
Data processing Pre-processing of raw data in GenomeStudio, Quantile normalization with no background subtraction, differential expression analysis using GeneSifter software
 
Submission date Oct 17, 2012
Last update date Mar 19, 2014
Contact name Ken Kikuchi
E-mail(s) kikuchi@ohsu.edu
Organization name Oregon Health & Science University
Department Pediatrics
Lab Keller
Street address 3181 S.W. Sam Jackson Park Road
City Portland
State/province OR
ZIP/Postal code 97239
Country USA
 
Platform ID GPL6885
Series (1)
GSE41675 Dynamic Pax3:Foxo1a expression of alveolar rhabdomyosarcoma

Data table header descriptions
ID_REF
VALUE Quantile normalized non-log transformed signal intensities generated during pre-processing using GenomeStudio
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 3219.7 1
ILMN_2721178 812.7 1
ILMN_3033922 1093 1
ILMN_3092673 3734 1
ILMN_2816356 193.9 0.99373
ILMN_2808939 2006.9 1
ILMN_2634564 1155.7 1
ILMN_2737647 121.4 0.70927
ILMN_2734484 1271.2 1
ILMN_2952292 265.6 1
ILMN_2699078 107.5 0.40476
ILMN_1213681 701.2 1
ILMN_2735413 96.7 0.08897
ILMN_2735415 122.8 0.72932
ILMN_2891688 802.3 1
ILMN_2637698 1988.4 1
ILMN_2674228 679 1
ILMN_2601546 123.3 0.74185
ILMN_1230831 137.9 0.901
ILMN_2848071 162.1 0.97118

Total number of rows: 25697

Table truncated, full table size 616 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap