|
| Status |
Public on Mar 19, 2014 |
| Title |
Experiment2_si_YFP_3N (S) |
| Sample type |
RNA |
| |
|
| Source name |
Mouse alveolar rhabdomyosarcoma tumor culture
|
| Organism |
Mus musculus |
| Characteristics |
Stage: S
tranfection: 10nM siYFP
genotype/variation: Myf6Cre,Pax3:Foxo1a,p53 conditional aRMS mouse
|
| Treatment protocol |
Cells were transfected by 10nM siYFP or control siRNA with 0.2% lipofectamine2000
|
| Growth protocol |
DMEM+10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Method of RNA isolation: Rnsasy Total RNA kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Ambion MessageAmp Premier
|
| |
|
| Hybridization protocol |
Illumina MouseRef-8 v 2.0 protocol
|
| Scan protocol |
Illumina MouseRef-8 v 2.0 protocol
|
| Description |
2siY3N
|
| Data processing |
Pre-processing of raw data in GenomeStudio, Quantile normalization with no background subtraction, differential expression analysis using GeneSifter software
|
| |
|
| Submission date |
Oct 17, 2012 |
| Last update date |
Mar 19, 2014 |
| Contact name |
Ken Kikuchi |
| E-mail(s) |
kikuchi@ohsu.edu
|
| Organization name |
Oregon Health & Science University
|
| Department |
Pediatrics
|
| Lab |
Keller
|
| Street address |
3181 S.W. Sam Jackson Park Road
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE41675 |
Dynamic Pax3:Foxo1a expression of alveolar rhabdomyosarcoma |
|
