|
| Status |
Public on Oct 31, 2012 |
| Title |
GFP-SAS siNon target |
| Sample type |
RNA |
| |
|
| Source name |
GFP-SAS, siNT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: GFP-SAS
cell type: squamous cell carcinoma cell line
treatment: siNT
|
| Treatment protocol |
GFP-SAS cells were treated with siNT, siAURKA-1 or -2 (10 nM), DMSO, or MLN8237 (100nM) for 24 h.
|
| Growth protocol |
GFP-SAS cells were incubated in a humidified atmosphere of 95% air and 5% CO2 at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
ISOGEN extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
10 ng of total RNA was labeled using a GeneAtlasTM 3'IVT Express Kit Assay (Affymetrix) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
7.5 µg fragmented and labeled aRNA were hybridized for 16h at 45℃ on Human Genome U219 Array Strips. Array Strips were washed and stained in the GeneAtlasTM Fluidics Station.
|
| Scan protocol |
Array Strips were scanned in the GeneAtlasTM imaging station.
|
| Description |
Tongue
|
| Data processing |
The robust multichip average (RMA) method was used for background correction and normalization using GeneSpring GX 12.1 software.
|
| |
|
| Submission date |
Oct 18, 2012 |
| Last update date |
Oct 31, 2012 |
| Contact name |
Hiroshi Tanaka |
| E-mail(s) |
tanachu3@m.ehime-u.ac.jp
|
| Organization name |
Ehime University
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
454 Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE41697 |
Microarray analysis of GFP-SAS cells treated with siAURKA and MLN8237 |
|
