|
| Status |
Public on Nov 01, 2013 |
| Title |
mouse lung tissue at week 4, 5Gy, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse lung tissue at week 4, 5Gy, biological rep3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung
strain: C57Bl/Ncr
treatment: 5 Gy radiation treated
time: week 4
gender: female
age: 10 wk
|
| Treatment protocol |
An age-matched cohort of mice received no ionizing radiation while additional cohorts received 5 Gy in a single dose or 17.5 Gy in a single dose.
|
| Growth protocol |
At 2, 4, 8, 16, and 30 weeks post-ionizing radiation samples of lung were collected in 3 mice at each radiation dose (including 0 Gy).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by cleaning on a Qiagen RNAeasy column.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse430_2 microarray. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip array scanner 3000 7 G.
|
| Description |
gene expression data from 5 Gy radiation treated mouse lung at 4 weeks, replicate 3
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA) using quantile normalization and reported in log2 scale.
|
| |
|
| Submission date |
Oct 23, 2012 |
| Last update date |
Nov 01, 2013 |
| Contact name |
Uma T Shankavaram |
| E-mail(s) |
uma@mail.nih.gov
|
| Phone |
301-496-6718
|
| Organization name |
NIH
|
| Department |
NCI
|
| Lab |
Radiation Oncology Branch
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE41789 |
Senescence gene signature of radiation fibrosis |
|
