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Sample GSM1024357 Query DataSets for GSM1024357
Status Public on Nov 01, 2013
Title mouse lung tissue at week 30, 0 Gy, biological rep2
Sample type RNA
 
Source name mouse lung tissue at week 30, 0 Gy, biological rep2
Organism Mus musculus
Characteristics tissue: lung
strain: C57Bl/Ncr
treatment: control
time: week 30
gender: female
age: 10 wk
Treatment protocol An age-matched cohort of mice received no ionizing radiation while additional cohorts received 5 Gy in a single dose or 17.5 Gy in a single dose.
Growth protocol At 2, 4, 8, 16, and 30 weeks post-ionizing radiation samples of lung were collected in 3 mice at each radiation dose (including 0 Gy).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by cleaning on a Qiagen RNAeasy column.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse430_2 microarray. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip array scanner 3000 7 G.
Description gene expression data from control mouse lung at 30 weeks, replicate 2
Data processing The data were analyzed with Robust Multichip Average (RMA) using quantile normalization and reported in log2 scale.
 
Submission date Oct 23, 2012
Last update date Nov 01, 2013
Contact name Uma T Shankavaram
E-mail(s) uma@mail.nih.gov
Phone 301-496-6718
Organization name NIH
Department NCI
Lab Radiation Oncology Branch
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL1261
Series (1)
GSE41789 Senescence gene signature of radiation fibrosis

Data table header descriptions
ID_REF
VALUE RMA log2 signal intensity

Data table
ID_REF VALUE
1415670_at 9.657108826
1415671_at 10.98125982
1415672_at 11.92967104
1415673_at 8.00161126
1415674_a_at 10.2265354
1415675_at 9.695946986
1415676_a_at 11.53223072
1415677_at 9.997484152
1415678_at 10.37228327
1415679_at 11.98206922
1415680_at 9.776260898
1415681_at 9.870471817
1415682_at 8.47381102
1415683_at 11.0943938
1415684_at 8.616606035
1415685_at 8.758661862
1415686_at 9.982037196
1415687_a_at 13.20254298
1415688_at 10.81235079
1415689_s_at 8.868326226

Total number of rows: 45037

Table truncated, full table size 1023 Kbytes.




Supplementary file Size Download File type/resource
GSM1024357_W30_control_50_madbID102418.cel.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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