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Status |
Public on Oct 26, 2012 |
Title |
LT-HSC Zbtb7a F/+ Mx1-Cre+ #2 |
Sample type |
RNA |
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Source name |
LT-HSCs of control mouse
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Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6 genotype/variation: Zbtb7a Flox/+ Mx1-Cre+ (LRF heterozygous) age: 4 weeks cell type: FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-) time point: 9 days after 1st pIpC injection
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Treatment protocol |
LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection. pIpC (250μg) was injected twice to induce Cre recombinase.
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Growth protocol |
N/A
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Extracted molecule |
total RNA |
Extraction protocol |
RNA samples were prepared using the Absolutely RNA Microprep Kit (Stratagene).
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Label |
biotin
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Label protocol |
Biotinylated cRNA was generated using two rounds of in vitro transcription by T7 RNA polymerase.
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Hybridization protocol |
Affymetrix GeneChip Mouse Genome 430 v2.0 arrays (Affymetrix, Santa Clara, CA) were hybridized with fragmented cRNA targets (20 μg) and washed and stained with streptavidin-PE.
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Scan protocol |
Affymetrix GeneChip images were scanned using a high-resolution GeneChip Scanner 3000 (Hewlett-Packard).
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Description |
B655_MG430 2-(2_2849F+) Gene expression data from FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-).
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Data processing |
The raw data is normalized by Robust Multi-array Average (RMA) using Affymetrix Expression Console.
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Submission date |
Oct 25, 2012 |
Last update date |
Oct 26, 2012 |
Contact name |
Takahiro Maeda |
E-mail(s) |
tmaeda@partners.org
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Organization name |
Brigham and Women's Hospital
|
Street address |
One Blackfan Circle
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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|
Platform ID |
GPL1261 |
Series (1) |
GSE41839 |
Expression data from control and LRF (leukemia/lymphoma related factor)-deficient mouse LT-HSCs |
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