|
| Status |
Public on Oct 26, 2012 |
| Title |
LT-HSC Zbtb7a F/F Mx1-Cre+ #3 |
| Sample type |
RNA |
| |
|
| Source name |
LT-HSCs of LRF knockout mouse
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: Zbtb7a Flox/Flox Mx1-Cre+ (LRF homozygous knockout)
age: 4 weeks
cell type: FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-)
time point: 9 days after 1st pIpC injection
|
| Treatment protocol |
LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection. pIpC (250μg) was injected twice to induce Cre recombinase.
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were prepared using the Absolutely RNA Microprep Kit (Stratagene).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was generated using two rounds of in vitro transcription by T7 RNA polymerase.
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Mouse Genome 430 v2.0 arrays (Affymetrix, Santa Clara, CA) were hybridized with fragmented cRNA targets (20 μg) and washed and stained with streptavidin-PE.
|
| Scan protocol |
Affymetrix GeneChip images were scanned using a high-resolution GeneChip Scanner 3000 (Hewlett-Packard).
|
| Description |
B658_MG430 2-(6_2851+2821FF)
Gene expression data from FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-).
|
| Data processing |
The raw data is normalized by Robust Multi-array Average (RMA) using Affymetrix Expression Console.
|
| |
|
| Submission date |
Oct 25, 2012 |
| Last update date |
Oct 26, 2012 |
| Contact name |
Takahiro Maeda |
| E-mail(s) |
tmaeda@partners.org
|
| Organization name |
Brigham and Women's Hospital
|
| Street address |
One Blackfan Circle
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE41839 |
Expression data from control and LRF (leukemia/lymphoma related factor)-deficient mouse LT-HSCs |
|
