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Sample GSM102720 Query DataSets for GSM102720
Status Public on Apr 07, 2006
Title WT E2, 24h vs. WT Vehicle control
Sample type RNA
 
Channel 1
Source name WT E2, 24h (Cy3)
Organism Mus musculus
Characteristics WT E2, 24h
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WT Vehicle control (Cy5)
Organism Mus musculus
Characteristics WT Vehicle control
Extracted molecule total RNA
Label Cy5
 
 
Description Mice were treated with sesame oil or E2 and uteri were collected 24 hours after treatment and snap frozen in liquid nitrogen. Three or four uteri from each treatment group were pooled and RNA was prepared using Trizol reagent and the RNeasy clean up protocol.
Data processing Five hundred ng of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer's protocol. For each two color comparison, 750 ng of each Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v7.5), using default for all parameters.
 
Submission date Mar 31, 2006
Last update date Jul 06, 2007
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL891
Series (1)
GSE4615 Estren Behaves as a Weak Estrogen Rather than a Non-genomic Selective Activator in the Mouse Uterus

Data table header descriptions
ID_REF
gProcessedSignal Cy3 signal (normalized, background subtracted)
rProcessedSignal Cy5 signal (normalized, background subtracted)
VALUE LogRatio (Log10, gProcessedSignal (Cy3):rProcessedSignal (Cy5))

Data table
ID_REF gProcessedSignal rProcessedSignal VALUE
1 1.05E+04 3.26E+03 0.508
2 2.36E+01 4.34E+01 -0
3 9.61E+02 8.12E+02 0.0733
4 1.04E+03 8.51E+02 0.0882
5 7.96E+03 2.62E+03 0.483
6 5.66E+02 3.55E+02 0.203
7 8.18E+03 2.99E+02 1.44
8 4.98E+02 3.53E+02 0.15
9 2.45E+02 1.92E+02 0.105
10 5.97E+02 3.58E+02 0.222
11 7.08E+03 6.25E+03 0.0545
12 7.92E+02 5.09E+02 0.192
13 3.19E+03 2.20E+03 0.162
14 8.80E+03 3.22E+02 1.44
15 9.93E+02 4.76E+02 0.319
16 3.82E+02 2.85E+02 0.127
17 7.70E+03 7.09E+03 0.0358
18 7.35E+03 4.22E+03 0.241
19 1.97E+04 8.85E+03 0.347
20 3.43E+03 2.76E+03 0.0951

Total number of rows: 22393

Table truncated, full table size 663 Kbytes.




Supplementary file Size Download File type/resource
GSM102720.tif.gz 24.4 Mb (ftp)(http) TIFF
GSM102720.txt.gz 5.2 Mb (ftp)(http) TXT

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