|
| Status |
Public on Nov 01, 2012 |
| Title |
CLP LY6D+ EBF1ko rep1 |
| Sample type |
RNA |
| |
|
| Source name |
CLP LY6D+ EBF1 ko
|
| Organism |
Mus musculus |
| Characteristics |
tissue type: bone marrow
genotype/variation: Ebf1 knock-out
facs-sorting: LIN(GR1/MAC1/TER119/CD3E/LY6C/NK1.1)-CD11C-CD19-FLT3hiIL7R+SCA1loKITloLY6D+
|
| Treatment protocol |
No treatment has been used.
|
| Growth protocol |
Cells obtained fresh from the bone marrow of mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Micro kit (Qiagen) has been used to extract RNA according to manufacturer instruction
|
| Label |
biotin
|
| Label protocol |
RNA was labelled and amplified according to Affymetrix TM GeneChip Expression Analysis Technical Manual 2011
|
| |
|
| Hybridization protocol |
cRNA fragments hybridized against MOE430 2.0 Affymetrix gene expression arrays chip according to manufacturer instructions
|
| Scan protocol |
Chips were scanned using a GeneChip TM Scanner 3000
|
| Description |
Expression profiling of LY6D- CLPs derived from Ebf1 deficient mice.
|
| Data processing |
Expression values were acquired using RMA and custom CDF files (Brainarray, ENTREZG_14.1.0).
|
| |
|
| Submission date |
Oct 31, 2012 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Robert Månsson |
| E-mail(s) |
robert.mansson@ki.se
|
| Organization name |
Lund University
|
| Department |
Hemapotoietic stem cell biology
|
| Street address |
Klinikgatan 26, BMC B12
|
| City |
Lund |
| ZIP/Postal code |
221 84 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL16225 |
| Series (1) |
| GSE41931 |
Expression profiling of early lymphoid progenitors deficient for Ebf1 and Foxo1 |
|
