|
Status |
Public on Nov 01, 2012 |
Title |
CLP LY6D+ FOXO1wt rep2 |
Sample type |
RNA |
|
|
Source name |
CLP LY6D+ FOXO1 wt
|
Organism |
Mus musculus |
Characteristics |
tissue type: bone marrow genotype/variation: wild-type facs-sorting: LIN(GR1/MAC1/TER119/CD3E/LY6C/NK1.1)-CD11C-CD19-FLT3hiIL7R+SCA1loKITloLY6D+
|
Treatment protocol |
No treatment has been used.
|
Growth protocol |
Cells obtained fresh from the bone marrow of mice.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Micro kit (Qiagen) has been used to extract RNA according to manufacturer instruction
|
Label |
biotin
|
Label protocol |
RNA was labelled and amplified according to Affymetrix TM GeneChip Expression Analysis Technical Manual 2011
|
|
|
Hybridization protocol |
cRNA fragments hybridized against MOE430 2.0 Affymetrix gene expression arrays chip according to manufacturer instructions
|
Scan protocol |
Chips were scanned using a GeneChip TM Scanner 3000
|
Description |
Expression profiling of LY6D- CLPs derived from Foxo1 wild-type mice.
|
Data processing |
Expression values were acquired using RMA and custom CDF files (Brainarray, ENTREZG_14.1.0).
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|
|
Submission date |
Oct 31, 2012 |
Last update date |
Nov 01, 2012 |
Contact name |
Robert MÃ¥nsson |
E-mail(s) |
robert.mansson@ki.se
|
Organization name |
Lund University
|
Department |
Hemapotoietic stem cell biology
|
Street address |
Klinikgatan 26, BMC B12
|
City |
Lund |
ZIP/Postal code |
221 84 |
Country |
Sweden |
|
|
Platform ID |
GPL16225 |
Series (1) |
GSE41931 |
Expression profiling of early lymphoid progenitors deficient for Ebf1 and Foxo1 |
|