|
| Status |
Public on Nov 01, 2012 |
| Title |
Surrounding 4 weeks rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Microdissected surrounding cells, infected
|
| Organism |
Mus musculus |
| Characteristics |
time: 4 wk
strain: C57BL/6
gender: Female
age: 6 weeks
tissue: surrounding cells outside the region of liver granulomas
infection: Schistosoma japonicum
|
| Treatment protocol |
Mice were euthanised and liver tissues were embedded in OCT compound to prepare frozen blocks for laser microdissection.
|
| Growth protocol |
The complete life cycle of schistosoma japonicum was maintained in our laboratory. The cercarial of S. japonicum were shed from Oncomelania hupensis snail to infect the experimental mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from microdissected materials was extracted by a combination use of Trizol (Invitrogen) and RNeasy Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
cRNA was synthesised from total RNA with Illumina Total Prep RNA Amplication kit (Ambion).
|
| |
|
| Hybridization protocol |
Synthesized cRNA was hybridised to Illumina mouse Ref-8 v2 expression beadchips according to manufacturer instructions.
|
| Scan protocol |
Images on the arrays were scanned using Beadstation 500 at green factor level 2.
|
| Description |
Gene expression of the surrounding cells outside the region of granulomas in the liver infected by Schistosoma japonicum at 4 weeks post infection
|
| Data processing |
Feature extraction of the data was generated using GenomeStudio version 1.1.1 and analysed further using GeneSpring GX 11.
|
| |
|
| Submission date |
Oct 31, 2012 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Geoffrey Gobert |
| E-mail(s) |
geoffG@qimr.edu.au
|
| Organization name |
QIMR
|
| Street address |
300 Herston Rd
|
| City |
Brisbane |
| ZIP/Postal code |
4006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE41941 |
Neutrophils play a key role in schistosome-induced hepatic granuloma formation |
|
