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Status |
Public on Jun 11, 2013 |
Title |
129S1/SvImJ_XX_E11.2_gonad_rep1 |
Sample type |
RNA |
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Source name |
129S1/SvImJ, XX, E11.2 Gonad
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Organism |
Mus musculus |
Characteristics |
strain: 129S1/SvImJ Sex: XX Stage: E11.2
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was first extracted from individual pairs of E11.0-E12.0 XX and XY gonads (separated from mesonephroi) with the RNeasy Micro kit with on-column DNase digestion (QIAGEN, cat no. 74004) following the manufacturer’s protocol. Total RNA was eluted in 14 ul RNase-free water (not DEPC- treated), and 2 ul were used to quantify RNA concentration on a NanoDrop ND-2000 (Thermo Scientific). Only samples with > 100 ng of total RNA and an A280:A260 ratio of >1.6 were included in the expression analyses.
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Label |
biotin
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Label protocol |
From each total RNA sample, mRNA was selectively reverse transcribed with oligo(dT) primers to T7-labelled cDNA, and then amplified by in vitro transcription (IVT) to produce biotinylated cRNA using the Illumina TotalPrep Amplification Kit (Ambion/ Life Technologies, cat no. AMIL1791) according to manufacturer’s instructions.
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Hybridization protocol |
Standard Illumina hybridization protocol
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Scan protocol |
Standard Illumina scanning protocol
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Description |
replicate 1
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Data processing |
The data were normalised using quantile normalisation with BeadArray,a package in BioConductor, in R [version of R 2.12.0 and beadarray version 2.0.6].
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Submission date |
Oct 31, 2012 |
Last update date |
Jun 11, 2013 |
Contact name |
Anirudh Natarajan |
Organization name |
Duke University Medical Center
|
Lab |
Blanche Capel
|
Street address |
4034 GSRBII Building, Research Drive
|
City |
Durham |
State/province |
NC |
ZIP/Postal code |
27705 |
Country |
USA |
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Platform ID |
GPL6885 |
Series (1) |
GSE41948 |
Fine Time-Course Expression Analysis of Mammalian Sex Determination in 129S1/SvImJ and C57BL/6J XX and XY mice |
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