NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM102847 Query DataSets for GSM102847
Status Public on Mar 26, 2007
Title Diseased 9
Sample type RNA
 
Channel 1
Source name motor cortex, reference
Organism Homo sapiens
Characteristics Sample ID: Diseased 2; Sample Type: tissue; Order Code: BTB3292 [Brain and Tissue Bank for Developmental Disorders (Miami, FL, USA)]; Chief Disease: ALS; Cause of Death: ALS; Age [years / days]: 76 / 197; Gender: Male; Race: Caucasian; PMI [h]: 24.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name motor cortex, diseased 9
Organism Homo sapiens
Characteristics Sample ID: Diseased 9; Sample Type: tissue; Order Code: BTB3689 [Brain and Tissue Bank for Developmental Disorders (Miami, FL, USA)]; Chief Disease: ALS; Cause of Death: n/a; Age [years / days]: 61 / 141; Gender: Female; Race: Caucasian; PMI [h]: 22.
Extracted molecule total RNA
Label Cy5
 
 
Description Complementary RNAs (cRNAs) labelled with Cy5-CTP (Perkin-Elmer) were synthesized from 1 µg of total RNA of each sample using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent Technologies) following the manufacturer’s protocol. A reference cRNA, labelled with Cy3-CTP (Perkin-Elmer), was synthesized from 1 µg of sample Diseased 2 RNA. Aliquots (750 ng) of Cy3 and Cy5 labelled cRNA targets were co-hybridized on Whole Human Genome Oligo Microarrays (Agilent Technologies). Microarray hybridization and washing were performed using reagents and instruments (hybridization chambers and rotating oven) as indicated by the manufacturer (Agilent Technologies). Microarrays were scanned at 10-μm resolution using a GenePix Personal 4100A microarray scanner and the GenePix Pro 6.0 acquisition and data-extraction software (Molecular Devices, Corp.).
Data processing No Normalization
 
Submission date Apr 03, 2006
Last update date Mar 26, 2007
Contact name Sebastiano Cavallaro
E-mail(s) s.cavallaro@isn.cnr.it
Phone +3909577262411
Fax +390957122426
URL http://web.tiscali.it/sebastiano_cavallaro/
Organization name Italian National Research Council
Department Institute of Neurological Sciences
Lab Functional Genomics Center
Street address Viale Regina Margherita, 6
City Catania
ZIP/Postal code 95123
Country Italy
 
Platform ID GPL1708
Series (1)
GSE4595 Expression profiling of motor cortex in sporadic amyotrophic lateral sclerosis

Data table header descriptions
ID_REF
VALUE Log2 Ratio (635/532)

Data table
ID_REF VALUE
3 -3.528
4 -2.459
5 -3.1
6 -3.916
8 -2.575
9 -2.939
10 -1.349
12 -1.77
13 -4.032
15 -2.888
16 -3.392
17 -3.511
18 -1.496
19 -1.238
20 -1.048
22 -2.265
23 -0.921
24 -1.989
25 -1.735
26 -3.476

Total number of rows: 39907

Table truncated, full table size 490 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap