|
| Status |
Public on Jun 01, 2013 |
| Title |
BCG_mouse1_BCGstim |
| Sample type |
RNA |
| |
|
| Source name |
Mouse splenocyte
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
strain: C57BL/6
age: 13 weeks
cell type: splenocyte
immunization: BCG
stimulation: BCG stimulation
|
| Growth protocol |
1.0E6 splenocytes for each sample were cultured for 12 hours at 37 degrees Celcius in RPMI medium with Hepes supplemented with 10% fetal calf serum and 2mM L-glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN Rneasy minikit including an on-column Dnase I digestion in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample name: 2.5
splenocytes were stimulated overnight with 1e6 CFU of BCG
|
| Data processing |
BeadStudio v2.0 used to generate the raw data (linked as supplementary file on Series record). Array quality assessment was performed using ArrayQualityMetrics prior to background correction and normalization using quantile normalisation with Bioconductor package limma, neqc function
|
| |
|
| Submission date |
Nov 06, 2012 |
| Last update date |
Jun 01, 2013 |
| Contact name |
leanne marsay |
| E-mail(s) |
Leanne.marsay@ndm.ox.ac.uk
|
| Phone |
01865 857445
|
| Organization name |
University of Oxford
|
| Department |
Nuffield department
|
| Lab |
Jenner Institute
|
| Street address |
roosevelt drive
|
| City |
Oxford |
| ZIP/Postal code |
OX4 3TU |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE42090 |
The innate and adaptive immune response to BCG stimulation in splenocytes taken from C57BL/6 mice |
|
