tissue: caudal and anal fins genotype: wild type developmental stage: adult (over 2 months old) cell type: Melanophore
Treatment protocol
Caudal and anal fins were collected from 1,000 adult zebrafish and were incubated in a trypsin solution containing 2.5 mg/ml trypsin, 1.2 mg/ml bovine serum albumin, and 1 mM EDTA for 1 overnight at 4℃. The fins were then washed with 1×PBS for 5mins at 1,000rpm five times at 28℃. After collagenase treatment melanophores and xanthophores were isolated as described in Inaba et al 2012.
Growth protocol
Zebrafish were obtained from pet shop.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using RNeasy mini kit (Qiagen, USA) according to the manufacturer's instructions.
Label
biotin
Label protocol
standard Affymetrix labeling, hybridization, scanning, and MAS 5.0 data processing were performed by outsourcing service in RIKEN CDB, Japan.
Hybridization protocol
standard Affymetrix labeling, hybridization, scanning, and MAS 5.0 data processing were performed by outsourcing service in RIKEN CDB, Japan.
Scan protocol
standard Affymetrix labeling, hybridization, scanning, and MAS 5.0 data processing were performed by outsourcing service in RIKEN CDB, Japan.
Data processing
standard Affymetrix labeling, hybridization, scanning, and MAS 5.0 data processing were performed by outsourcing service in RIKEN CDB, Japan.
