|
| Status |
Public on Nov 01, 2015 |
| Title |
SHSY5Y cells_Gelsemium 2c_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
SHSY5Y cells_Gelsemium 2c
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SHSY5Y (ATCC N. CRL-2266)
cell type: neuroblastoma
|
| Growth protocol |
Neuroblastoma cells adherent onto Petri dishes at standard density were collected after mild treatment with Trypsin-PBS solution (5 mg/L).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 3.5 x 106 cells with Qiagen RNAeasy Mini Kit (Qiagen S.p.A., Milan, Italy) following the manufacturer’s instructions (Animal cells Spin protocol), including genomic DNA elimination step in column. The qualitative parameters A260/A280>1.8, A260/A230>1.7; RNA integrity number>7 were evaluated.
|
| Label |
Cy3
|
| Label protocol |
cDNA synthesis and labeling reactions were performed according to the NimbleGen Arrays User’s Guide (V 5.1).
|
| |
|
| Hybridization protocol |
Hybridization and washing procedures were performed according to the NimbleGen Arrays User’s Guide (V 5.1).
|
| Scan protocol |
Each microarray was scanned using an Axon GenePix 4400 A at 532 nm and GenePix Pro7 software (Molecular Devices, Sunnyvale, CA, USA).
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction.
|
| |
|
| Submission date |
Nov 13, 2012 |
| Last update date |
Nov 01, 2015 |
| Contact name |
Alberto Ferrarini |
| E-mail(s) |
alberto.ferrarini@univr.it
|
| Phone |
+39-045-802-7058
|
| Organization name |
University of Verona
|
| Department |
Scientific and Technological Department
|
| Lab |
Plant Functional Genomics Centre
|
| Street address |
Strada le Grazie, 15
|
| City |
Verona |
| State/province |
Veneto |
| ZIP/Postal code |
37134 |
| Country |
Italy |
| |
|
| Platform ID |
GPL16025 |
| Series (1) |
| GSE42236 |
Extreme sensitivity of gene expression in human neurocytes SH-SY5Y to ultra-low doses of a medicinal plant with anxiolytic-like properties |
|
