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Sample GSM1037933 Query DataSets for GSM1037933
Status Public on Sep 03, 2013
Title Adipocytes_physiological saline_replicate 2
Sample type RNA
 
Source name 3T3-L1 cultured adipocytes treated with physiological saline
Organism Mus musculus
Characteristics cell line: 3T3-L1
treatment: physiological saline
Treatment protocol Mature adipocytes were treated with different types of asbestos fibers at a concentration of 10 mg/cm2. After 72 hours of incubation, total RNA was isolated from the cells. Physiological saline was used as a control.
Growth protocol Mature adipocytes were differentiated from 3T3-L1 preadipocyte cell line. After differentiation, mature adipocytes were maintained in DMEM medium supplemented with 10% fetal bovine serum and 1% antibiotic-antimycotic (Gibco 15240). The cells were maintained in an incubator with 5% CO2 at 37oC.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using RNeasy Mini(QIAGEN).
Label Cy3
Label protocol According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling, Version 6.5, May 2010).
 
Hybridization protocol According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling, Version 6.5, May 2010).
Scan protocol According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling, Version 6.5, May 2010, Agilent C Scanner Settings).
Data processing The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 028005_D_F_20110722) to obtain background subtracted and spatially detrended Processed Signal intensities.
 
Submission date Nov 15, 2012
Last update date Sep 03, 2013
Contact name Shinya Toyokuni
E-mail(s) akatsuka@med.nagoya-u.ac.jp
Organization name Nagoya University
Department Pathology
Street address 65 Tsuruma-Cho, Showa-Ku
City Nagoya
State/province Aichi
ZIP/Postal code 466-8550
Country Japan
 
Platform ID GPL13912
Series (1)
GSE42330 Gene expression analysis of cultured adipocytes treated with different types of asbestos fibers

Data table header descriptions
ID_REF
VALUE processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
ID_REF VALUE
1 1.688582e+004
2 5.089261e+000
3 3.630993e+000
4 3.647463e+000
5 8.115945e+001
6 3.679727e+000
7 1.633474e+001
8 2.876853e+003
9 5.005083e+000
10 3.731332e+000
11 2.079455e+001
12 9.843637e+000
13 2.607313e+002
14 6.393872e+003
15 1.559683e+001
16 3.775757e+000
17 2.694963e+003
18 2.209924e+002
19 1.725792e+003
20 4.285361e+004

Total number of rows: 62976

Table truncated, full table size 1219 Kbytes.




Supplementary file Size Download File type/resource
GSM1037933_Saline_2.txt.gz 11.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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