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Status |
Public on Sep 03, 2013 |
Title |
Adipocytes_physiological saline_replicate 2 |
Sample type |
RNA |
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Source name |
3T3-L1 cultured adipocytes treated with physiological saline
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Organism |
Mus musculus |
Characteristics |
cell line: 3T3-L1 treatment: physiological saline
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Treatment protocol |
Mature adipocytes were treated with different types of asbestos fibers at a concentration of 10 mg/cm2. After 72 hours of incubation, total RNA was isolated from the cells. Physiological saline was used as a control.
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Growth protocol |
Mature adipocytes were differentiated from 3T3-L1 preadipocyte cell line. After differentiation, mature adipocytes were maintained in DMEM medium supplemented with 10% fetal bovine serum and 1% antibiotic-antimycotic (Gibco 15240). The cells were maintained in an incubator with 5% CO2 at 37oC.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using RNeasy Mini(QIAGEN).
|
Label |
Cy3
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Label protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling, Version 6.5, May 2010).
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Hybridization protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling, Version 6.5, May 2010).
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Scan protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis, Low Input Quick Amp Labeling, Version 6.5, May 2010, Agilent C Scanner Settings).
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Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 028005_D_F_20110722) to obtain background subtracted and spatially detrended Processed Signal intensities.
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Submission date |
Nov 15, 2012 |
Last update date |
Sep 03, 2013 |
Contact name |
Shinya Toyokuni |
E-mail(s) |
akatsuka@med.nagoya-u.ac.jp
|
Organization name |
Nagoya University
|
Department |
Pathology
|
Street address |
65 Tsuruma-Cho, Showa-Ku
|
City |
Nagoya |
State/province |
Aichi |
ZIP/Postal code |
466-8550 |
Country |
Japan |
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|
Platform ID |
GPL13912 |
Series (1) |
GSE42330 |
Gene expression analysis of cultured adipocytes treated with different types of asbestos fibers |
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