|
| Status |
Public on Dec 15, 2014 |
| Title |
lung_WT_6303_High_6hrs_1-3 |
| Sample type |
RNA |
| |
|
| Source name |
WT lung, ATCC6303 high dose infected, Asc experimental day, 6 hours
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
strain: C57BL6/J
tissue: lung
|
| Treatment protocol |
Lungs homogenated in PBS prior to RNA isolation
|
| Growth protocol |
S. pneumoniae serotype 2 D39 strain and serotype 3 ATCC6303 strain were grown to mid-logarithmic phase at 37°C using Todd–Hewitt broth (Difco, Detroit, MI) supplemented with yeast extract (0.5%), harvested by centrifugation at 4000rpm for 15 min, and washed twice in isotonic saline. Suspensions of 2x107 (high dose) and 5x104 (low dose) colony forming units (CFU) in 50µL isotonic saline were used as the inocula.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Nucleospin protocol
|
| Label |
biotin
|
| Label protocol |
Illumina standard protocol
|
| |
|
| Hybridization protocol |
Illumina standard protocol
|
| Scan protocol |
Illumina standard protocol
|
| Description |
6331505017_E
|
| Data processing |
NEQC normalized
R statistical environment packages beadarray and limma
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Dec 15, 2014 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
|
| Organization name |
University of Malta
|
| Street address |
Msida campus
|
| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE42464 |
Lung gene expression analysis of WT, Nlrp3-, Asc-deficient mice in Streptococcus pneumoniae D39 and ATCC6303 infection |
|
