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Sample GSM1040644 Query DataSets for GSM1040644
Status Public on Dec 15, 2014
Title lung_WT_3
Sample type RNA
 
Source name WT lung, uninfected
Organism Mus musculus
Characteristics gender: female
strain: C57BL6/J
tissue: lung
Treatment protocol Lungs homogenated in PBS prior to RNA isolation
Growth protocol S. pneumoniae serotype 2 D39 strain and serotype 3 ATCC6303 strain were grown to mid-logarithmic phase at 37°C using Todd–Hewitt broth (Difco, Detroit, MI) supplemented with yeast extract (0.5%), harvested by centrifugation at 4000rpm for 15 min, and washed twice in isotonic saline. Suspensions of 2x107 (high dose) and 5x104 (low dose) colony forming units (CFU) in 50µL isotonic saline were used as the inocula.
Extracted molecule total RNA
Extraction protocol Nucleospin protocol
Label biotin
Label protocol Illumina standard protocol
 
Hybridization protocol Illumina standard protocol
Scan protocol Illumina standard protocol
Description 6331441017_C
Data processing NEQC normalized
R statistical environment packages beadarray and limma
 
Submission date Nov 21, 2012
Last update date Dec 15, 2014
Contact name Brendon Scicluna
E-mail(s) brendon.scicluna@um.edu.mt
Organization name University of Malta
Street address Msida campus
City Msida
ZIP/Postal code MSD2020
Country Malta
 
Platform ID GPL6885
Series (1)
GSE42464 Lung gene expression analysis of WT, Nlrp3-, Asc-deficient mice in Streptococcus pneumoniae D39 and ATCC6303 infection

Data table header descriptions
ID_REF
VALUE normalized signal intensity
detection P value

Data table
ID_REF VALUE detection P value
ILMN_1250052 5.763521971 0
ILMN_3122480 5.087909537 0.177295918
ILMN_2599935 7.982661421 0
ILMN_2675543 4.7879972 0.566326531
ILMN_2686883 6.187651264 0
ILMN_2751818 4.665599121 0.80994898
ILMN_2728634 6.245014845 0
ILMN_3040515 4.664549324 0.8125
ILMN_2711608 5.248428305 0.096938776
ILMN_1232875 5.923500239 0
ILMN_1258507 5.839014655 0
ILMN_2746142 4.552360327 0.945153061
ILMN_1252690 5.251361501 0.094387755
ILMN_2655499 4.790686723 0.558673469
ILMN_1252870 5.22049218 0.100765306
ILMN_1248179 4.75333017 0.633928571
ILMN_2649955 6.170425693 0
ILMN_2628708 6.037736552 0
ILMN_3024781 4.866538542 0.43494898
ILMN_2705628 7.868027282 0

Total number of rows: 25681

Table truncated, full table size 786 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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