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Sample GSM104433 Query DataSets for GSM104433
Status Public on Dec 01, 2008
Title WK1-placebo vs. WK1-E (REP1, HYB1)
Sample type RNA
 
Channel 1
Source name WK1-placebo (Cy3)
Organism Mus musculus
Characteristics WK1-placebo
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name WK1-E (Cy5)
Organism Mus musculus
Characteristics WK1-E
Extracted molecule total RNA
Label Cy5
 
 
Description Frozen mammary gland tissue, collected from four to five mice at day seven (placebo) or day seven (estradiol), was pooled and RNA was prepared using Trizol reagent and the RNeasy clean up protocol.
Data processing Five hundred ng of total RNA was amplified and labeled using the Agilent Low RNA Input Fluorescent Linear Amplification Kit according to the manufacturer's protocol. For each two color comparison, 750 ng of each Cy3- and Cy5-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 17 hours in a rotating hybridization oven according to the Agilent 60-mer oligo microarray processing protocol prior to washing and scanning with an Agilent Scanner. Data was obtained using the Agilent Feature Extraction software (v7.5), using default for all parameters.
 
Submission date Apr 10, 2006
Last update date Aug 22, 2008
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL891
Series (1)
GSE4647 Profile of estrogen-responsive genes in an estrogen-specific mammary gland outgrowth model

Data table header descriptions
ID_REF
gProcessedSignal Cy3 signal (normalized, background subtracted)
rProcessedSignal Cy5 signal (normalized, background subtracted)
VALUE LogRatio (Log10, rProcessedSignal (Cy5):gProcessedSignal (Cy3))

Data table
ID_REF gProcessedSignal rProcessedSignal VALUE
1 1.14E+04 4.92E+02 -1.37E+00
2 1.34E+02 3.40E+01 -5.96E-01
3 2.87E+02 2.42E+02 -7.39E-02
4 2.20E+02 1.57E+02 -1.45E-01
5 1.53E+04 1.32E+04 -6.55E-02
6 4.16E+02 3.10E+02 -1.27E-01
7 1.12E+04 4.85E+01 -2.00E+00
8 2.72E+02 2.80E+02 1.34E-02
9 9.34E+01 7.16E+01 -1.15E-01
10 2.88E+02 2.70E+02 -2.86E-02
11 3.52E+03 3.79E+03 3.09E-02
12 3.60E+02 3.19E+02 -5.27E-02
13 7.37E+02 6.08E+02 -8.31E-02
14 1.25E+04 1.77E+02 -1.85E+00
15 1.44E+03 1.79E+03 9.38E-02
16 2.66E+02 1.77E+02 -1.77E-01
17 9.03E+02 9.81E+02 3.59E-02
18 4.32E+03 5.35E+03 9.30E-02
19 2.16E+04 2.12E+04 -8.42E-03
20 1.11E+03 1.03E+03 -3.19E-02

Total number of rows: 22393

Table truncated, full table size 721 Kbytes.




Supplementary file Size Download File type/resource
GSM104433.tif.gz 25.4 Mb (ftp)(http) TIFF
GSM104433.txt.gz 5.2 Mb (ftp)(http) TXT

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