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Sample GSM1044355 Query DataSets for GSM1044355
Status Public on Jun 01, 2013
Title SVR, 50 micromolar propranolol, 4h (rep 1)
Sample type RNA
 
Source name 50 micromolar propranolol, 4h
Organism Mus musculus
Characteristics cell line: SVR
cell type: angiosarcoma
Treatment protocol Cells were treated with sham (t=0 hrs) or 50 micromolar propranolol (t=4 & 24 hrs)
Growth protocol SVR cells were cultured to 100% confluence in T75 flasks in Dulbecco's modified Eagle's media (DMEM) supplemented with 10% fetal bovine serum (FBS), 80 U/ml penicillin, and 50 micrograms/ml streptomycin C.
Extracted molecule total RNA
Extraction protocol RNA was purified using the Pulink RNA mini kit (Ambion) according to manufacturer's instructions. Quality control was performed with the Nanodrop and Bioanalyzer.
Label biotin
Label protocol Total RNA was amplified and biotin-labeled using Illumina TotalPrep RNA Amplification Kit (Ambion).
 
Hybridization protocol 750 ng of biotinylated aRNA was then briefly heat-denatured and loaded onto Illumina expression arrays to hybridize overnight.
Scan protocol Illumina ISCAN.
Description 8090936018_D
Data processing Intensity values were transferred to Agilent GeneSpring GX microarray analysis software and data was filtered based on quality of each call.
 
Submission date Nov 27, 2012
Last update date Jun 01, 2013
Contact name Brad Allen Bryan
E-mail(s) brad.bryan@ttuhsc.edu
Organization name Texas Tech University Health Sciences Center
Department Biomedical Sciences
Street address 5001 El Paso Drive
City El Paso
State/province Texas
ZIP/Postal code 79905
Country USA
 
Platform ID GPL6885
Series (1)
GSE42534 Genome-wide analysis of the effects of propranolol on angiosarcoma tumor cells

Data table header descriptions
ID_REF
VALUE Quantile normalized.
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 88.74779 0.3170426
ILMN_2707227 98.67533 0.02005013
ILMN_2896528 1379.218 0
ILMN_2721178 508.1322 0
ILMN_1227723 419.839 0
ILMN_3033922 546.2715 0
ILMN_3092673 1459.863 0
ILMN_2730714 2610.892 0
ILMN_3162224 1394.85 0
ILMN_2816356 104.0681 0.01002506
ILMN_2808939 1075.047 0
ILMN_2634564 625.0382 0
ILMN_1216623 577.2352 0
ILMN_1215157 814.7749 0
ILMN_1228777 79.5556 0.9837093
ILMN_2737647 88.77325 0.3132832
ILMN_1216425 85.14764 0.6679198
ILMN_2734484 126.157 0
ILMN_2652961 86.2767 0.5488722
ILMN_2952292 128.9059 0

Total number of rows: 25697

Table truncated, full table size 732 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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