|
| Status |
Public on Jul 01, 2013 |
| Title |
old islets at G3, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Old mice islets cultured at 3mM of glucose
|
| Organism |
Mus musculus |
| Characteristics |
tissue: pancreatic islets
strain: C57BL6 mice
age: 56-week-old
|
| Treatment protocol |
Islets were cultured in supplemented RPMI medium for two days at different glucose concentrations (3 mM, 5.5 mM, 11mM, and 16 mM) before RNA extraction
|
| Growth protocol |
Mouse pancreatic islets were isolated from wild-type C57/Bl6J male mice. Islets were allowed to recover for 1 day in RPMI (Invitrogen) containing 11mM glucose supplemented with 10% FCS and penicillin/streptomycin (Invitrogen) at 37°C, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol through two cycles of cDNA synthesis (50 ng starting total RNA).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized, washed and stained according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
|
| Description |
Gene expression data from old islets at 3mM glucose
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Nov 28, 2012 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Joan Marc Servitja |
| E-mail(s) |
servitja@clinic.ub.es
|
| Organization name |
IDIBAPS
|
| Street address |
Rosselló, 153
|
| City |
Barcelona |
| State/province |
Catalonia |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE42591 |
Expression data from fresh and cultured islets at different glucose concentrations |
|
