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Sample GSM1045557 Query DataSets for GSM1045557
Status Public on Jul 01, 2013
Title old islets at G5, biological rep2
Sample type RNA
 
Source name Old mice islets cultured at 5.5mM of glucose
Organism Mus musculus
Characteristics tissue: pancreatic islets
strain: C57BL6 mice
age: 56-week-old
Treatment protocol Islets were cultured in supplemented RPMI medium for two days at different glucose concentrations (3 mM, 5.5 mM, 11mM, and 16 mM) before RNA extraction
Growth protocol Mouse pancreatic islets were isolated from wild-type C57/Bl6J male mice. Islets were allowed to recover for 1 day in RPMI (Invitrogen) containing 11mM glucose supplemented with 10% FCS and penicillin/streptomycin (Invitrogen) at 37°C, 5% CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol through two cycles of cDNA synthesis (50 ng starting total RNA).
 
Hybridization protocol Following fragmentation, cRNA were hybridized, washed and stained according to the standard Affymetrix protocol.
Scan protocol GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
Description Gene expression data from old islets at 5.5mM glucose
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Nov 28, 2012
Last update date Jul 01, 2013
Contact name Joan Marc Servitja
E-mail(s) servitja@clinic.ub.es
Organization name IDIBAPS
Street address Rosselló, 153
City Barcelona
State/province Catalonia
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL1261
Series (1)
GSE42591 Expression data from fresh and cultured islets at different glucose concentrations

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 1230 P 2.44E-04
1415671_at 1621 P 2.44E-04
1415672_at 1731 P 2.44E-04
1415673_at 442 P 7.32E-04
1415674_a_at 578 P 2.44E-04
1415675_at 649 P 2.44E-04
1415676_a_at 870 P 2.44E-04
1415677_at 144 P 1.22E-03
1415678_at 867 P 2.44E-04
1415679_at 2008 P 2.44E-04
1415680_at 175 P 2.44E-04
1415681_at 404 P 2.44E-04
1415682_at 220 P 2.44E-04
1415683_at 694 P 2.44E-04
1415684_at 383 P 2.44E-04
1415685_at 543 P 2.44E-04
1415686_at 931 P 2.44E-04
1415687_a_at 4464 P 2.44E-04
1415688_at 794 P 2.44E-04
1415689_s_at 524 P 2.44E-04

Total number of rows: 45101

Table truncated, full table size 1124 Kbytes.




Supplementary file Size Download File type/resource
GSM1045557.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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