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Sample GSM1045561 Query DataSets for GSM1045561
Status Public on Jul 01, 2013
Title fresh old islets, biological rep2
Sample type RNA
 
Source name Fresh old mice islets
Organism Mus musculus
Characteristics tissue: pancreatic islets
strain: C57BL6 mice
age: 56-week-old
Treatment protocol Islets were cultured in supplemented RPMI medium for two days at different glucose concentrations (3 mM, 5.5 mM, 11mM, and 16 mM) before RNA extraction
Growth protocol Mouse pancreatic islets were isolated from wild-type C57/Bl6J male mice. Islets were allowed to recover for 1 day in RPMI (Invitrogen) containing 11mM glucose supplemented with 10% FCS and penicillin/streptomycin (Invitrogen) at 37°C, 5% CO2.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol through two cycles of cDNA synthesis (50 ng starting total RNA).
 
Hybridization protocol Following fragmentation, cRNA were hybridized, washed and stained according to the standard Affymetrix protocol.
Scan protocol GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.0.0.1214, scanner GCS 3000-7G)
Description Gene expression data from fresh old islets
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Nov 28, 2012
Last update date Jul 01, 2013
Contact name Joan Marc Servitja
E-mail(s) servitja@clinic.ub.es
Organization name IDIBAPS
Street address Rosselló, 153
City Barcelona
State/province Catalonia
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL1261
Series (1)
GSE42591 Expression data from fresh and cultured islets at different glucose concentrations

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 1337 P 2.44E-04
1415671_at 1667 P 2.44E-04
1415672_at 1762 P 2.44E-04
1415673_at 155 P 4.15E-03
1415674_a_at 512 P 2.44E-04
1415675_at 597 P 2.44E-04
1415676_a_at 817 P 2.44E-04
1415677_at 165 P 1.95E-03
1415678_at 710 P 2.44E-04
1415679_at 1723 P 2.44E-04
1415680_at 215 P 7.32E-04
1415681_at 382 P 2.44E-04
1415682_at 215 P 2.44E-04
1415683_at 569 P 2.44E-04
1415684_at 358 P 2.44E-04
1415685_at 439 P 2.44E-04
1415686_at 880 P 2.44E-04
1415687_a_at 3382 P 2.44E-04
1415688_at 675 P 2.44E-04
1415689_s_at 572 P 2.44E-04

Total number of rows: 45101

Table truncated, full table size 1126 Kbytes.




Supplementary file Size Download File type/resource
GSM1045561.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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