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Sample GSM104611 Query DataSets for GSM104611
Status Public on Jul 14, 2006
Title 15 min_IVS Sham tissue, biological rep1, Bv2
Sample type RNA
 
Source name C57/BL6 heart tissue
Organism Mus musculus
Characteristics Weight of mouse: 25-30g
Age: 8-10 wk old
Treatment protocol Permanent occlusion of the Left Anterior Descending coronary artery was performed on adult male (8 to 10 wk old) C57BL/6J mice. To exclude surgical effects on gene expression, sham surgeries were conducted by passing suture under the artery without ligation. Animals were sacrificed at time t=0 (naïve control) and at each time point post-AMI (0.25, 1, 4, 12, 24 and 48 h)
At each time point 4-10 animals were sacrificed and hearts harvested, regionally dissected and immediately frozen in liquid N2.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from individual frozen tissue samples using an SDS/phenol isolation protocol after which equal weights of RNA from 4-10 mice were pooled for each dissected tissue type.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from different regions of the mouse heart
Data processing The data were analyzed with Robust Multi-array Averaging (RMA; www.bioconductor.org) and global scaling (across 192 chips)as normalization method.
 
Submission date Apr 10, 2006
Last update date Jul 11, 2006
Contact name Mark Stayton
E-mail(s) stayton@uwyo.edu
Phone 307 766-4025
Fax 307 766-5098
Organization name University of Wyoming
Department Molecular Biology
Street address Dept. 3944, 1000 E. University Ave.
City Laramie
State/province WY
ZIP/Postal code 82071
Country USA
 
Platform ID GPL82
Series (1)
GSE4648 Earliest Changes in the Left Ventricular Transcriptome Post-Myocardial Infarction

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
104769_at 14.58672397
104770_at 41.02942412
104771_at 32.29271869
104772_at 65.20700264
104773_at 8.780237717
104774_at 14.55189366
104775_at 74.59530885
104776_at 27.40961198
104777_at 8.404622377
104778_at 26.74423441
104779_at 145.949049
104780_at 29.1935955
104781_at 16.14724352
104782_at 34.55273486
104783_at 32.49304403
104784_at 18.3210718
104785_at 27.70040184
104786_at 14.91348994
104787_at 41.17908875
104788_at 120.5912149

Total number of rows: 12477

Table truncated, full table size 270 Kbytes.




Supplementary file Size Download File type/resource
GSM104611.CEL.gz 2.8 Mb (ftp)(http) CEL

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