|
| Status |
Public on Jul 14, 2006 |
| Title |
60 min_post-AMI_FW tissue, biological rep2, Cv2 |
| Sample type |
RNA |
| |
|
| Source name |
C57/BL6 heart tissue
|
| Organism |
Mus musculus |
| Characteristics |
Weight of mouse: 25-30g
Age: 8-10 wk old
|
| Treatment protocol |
Permanent occlusion of the Left Anterior Descending coronary artery was performed on adult male (8 to 10 wk old) C57BL/6J mice. To exclude surgical effects on gene expression, sham surgeries were conducted by passing suture under the artery without ligation. Animals were sacrificed at time t=0 (naïve control) and at each time point post-AMI (0.25, 1, 4, 12, 24 and 48 h)
At each time point 4-10 animals were sacrificed and hearts harvested, regionally dissected and immediately frozen in liquid N2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from individual frozen tissue samples using an SDS/phenol isolation protocol after which equal weights of RNA from 4-10 mice were pooled for each dissected tissue type.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from different regions of the mouse heart
|
| Data processing |
The data were analyzed with Robust Multi-array Averaging (RMA; www.bioconductor.org) and global scaling (across 192 chips)as normalization method.
|
| |
|
| Submission date |
Apr 10, 2006 |
| Last update date |
Jul 11, 2006 |
| Contact name |
Mark Stayton |
| E-mail(s) |
stayton@uwyo.edu
|
| Phone |
307 766-4025
|
| Fax |
307 766-5098
|
| Organization name |
University of Wyoming
|
| Department |
Molecular Biology
|
| Street address |
Dept. 3944, 1000 E. University Ave.
|
| City |
Laramie |
| State/province |
WY |
| ZIP/Postal code |
82071 |
| Country |
USA |
| |
|
| Platform ID |
GPL83 |
| Series (1) |
| GSE4648 |
Earliest Changes in the Left Ventricular Transcriptome Post-Myocardial Infarction |
|
