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Sample GSM104701 Query DataSets for GSM104701
Status Public on Jul 14, 2006
Title Naïve IVS, biological rep2, Bv2
Sample type RNA
 
Source name C57/BL6 heart tissue
Organism Mus musculus
Characteristics Weight of mouse: 25-30g
Age: 8-10 wk old
Treatment protocol Permanent occlusion of the Left Anterior Descending coronary artery was performed on adult male (8 to 10 wk old) C57BL/6J mice. To exclude surgical effects on gene expression, sham surgeries were conducted by passing suture under the artery without ligation. Animals were sacrificed at time t=0 (naïve control) and at each time point post-AMI (0.25, 1, 4, 12, 24 and 48 h)
At each time point 4-10 animals were sacrificed and hearts harvested, regionally dissected and immediately frozen in liquid N2.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from individual frozen tissue samples using an SDS/phenol isolation protocol after which equal weights of RNA from 4-10 mice were pooled for each dissected tissue type.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from different regions of the mouse heart
Data processing The data were analyzed with Robust Multi-array Averaging (RMA; www.bioconductor.org) and global scaling (across 192 chips)as normalization method.
 
Submission date Apr 10, 2006
Last update date Jul 11, 2006
Contact name Mark Stayton
E-mail(s) stayton@uwyo.edu
Phone 307 766-4025
Fax 307 766-5098
Organization name University of Wyoming
Department Molecular Biology
Street address Dept. 3944, 1000 E. University Ave.
City Laramie
State/province WY
ZIP/Postal code 82071
Country USA
 
Platform ID GPL82
Series (1)
GSE4648 Earliest Changes in the Left Ventricular Transcriptome Post-Myocardial Infarction

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
104769_at 13.9286835
104770_at 48.44399706
104771_at 38.4948088
104772_at 48.83135818
104773_at 8.092766668
104774_at 18.79002394
104775_at 86.9303471
104776_at 21.48174075
104777_at 8.518987658
104778_at 28.30543127
104779_at 152.746234
104780_at 21.46182715
104781_at 14.66943092
104782_at 33.83327666
104783_at 42.39493475
104784_at 17.53108036
104785_at 31.31886446
104786_at 13.72204883
104787_at 40.15127789
104788_at 163.297512

Total number of rows: 12477

Table truncated, full table size 270 Kbytes.




Supplementary file Size Download File type/resource
GSM104701.CEL.gz 2.7 Mb (ftp)(http) CEL

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