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Sample GSM104722 Query DataSets for GSM104722
Status Public on Jul 14, 2006
Title 60 min_IVS Sham tissue, biological rep2, Cv2
Sample type RNA
 
Source name C57/BL6 heart tissue
Organism Mus musculus
Characteristics Weight of mouse: 25-30g
Age: 8-10 wk old
Treatment protocol Permanent occlusion of the Left Anterior Descending coronary artery was performed on adult male (8 to 10 wk old) C57BL/6J mice. To exclude surgical effects on gene expression, sham surgeries were conducted by passing suture under the artery without ligation. Animals were sacrificed at time t=0 (naïve control) and at each time point post-AMI (0.25, 1, 4, 12, 24 and 48 h)
At each time point 4-10 animals were sacrificed and hearts harvested, regionally dissected and immediately frozen in liquid N2.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from individual frozen tissue samples using an SDS/phenol isolation protocol after which equal weights of RNA from 4-10 mice were pooled for each dissected tissue type.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from different regions of the mouse heart
Data processing The data were analyzed with Robust Multi-array Averaging (RMA; www.bioconductor.org) and global scaling (across 192 chips)as normalization method.
 
Submission date Apr 10, 2006
Last update date Jul 11, 2006
Contact name Mark Stayton
E-mail(s) stayton@uwyo.edu
Phone 307 766-4025
Fax 307 766-5098
Organization name University of Wyoming
Department Molecular Biology
Street address Dept. 3944, 1000 E. University Ave.
City Laramie
State/province WY
ZIP/Postal code 82071
Country USA
 
Platform ID GPL83
Series (1)
GSE4648 Earliest Changes in the Left Ventricular Transcriptome Post-Myocardial Infarction

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
128520_at 739.210275
128523_at 6.994054316
128529_at 20.39756454
128550_at 82.8597177
128554_at 37.69904311
128577_s_at 17.68412583
128578_r_at 20.68412466
128579_r_at 38.35633579
128580_at 69.68146255
128582_at 13.47568775
128583_at 16.41241916
128590_at 42.96699568
128624_at 15.54265434
128643_r_at 360.8203756
128644_at 214.6454826
128645_at 59.03035038
128646_at 19.67140366
128647_at 45.08526199
128651_at 110.2841002
128652_at 26.55535664

Total number of rows: 11934

Table truncated, full table size 264 Kbytes.




Supplementary file Size Download File type/resource
GSM104722.CEL.gz 2.8 Mb (ftp)(http) CEL

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